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Development and Optimization of Novel Emulgel Loaded with Andrographolide-Rich Extract and Sesame Oil Using Quality
N V L Sirisha Mulukuri1, Moumita Dhara2, Dheeraj Gupta1
1Department of Pharmaceutical Chemistry, NGSM Institute of Pharmaceutical Sciences (NGSMIPS), Nitte (Deemed to be University), Mangalore 575018, India.
Gels (Basel, Switzerland)
|July 28, 2023
Summary
This study developed a nanoemulgel of andrographolide-rich extract to enhance its skin cancer treatment efficacy. The optimized nanoemulgel effectively inhibited epidermoid carcinoma cell growth and induced cell cycle arrest, showing promise for anti-skin-cancer therapy.
Area of Science:
- Dermatology and Oncology
- Nanotechnology in Drug Delivery
- Natural Product Chemistry
Background:
- Epidermoid carcinoma is a non-melanoma skin cancer.
- Andrographis extract and andrographolide show anticancer properties.
- Improved delivery systems are needed to enhance efficacy.
Purpose of the Study:
- To formulate and optimize a nanoemulgel of andrographolide-rich extract.
- To evaluate the anticancer potential of the nanoemulgel against epidermoid carcinoma cells.
- To assess the safety and mechanism of action of the optimized formulation.
Main Methods:
- Formulation of emulgels using sonication and homogenization.
- Optimization using a 2^2-factorial design and Quality by Design (QbD).
- Characterization via particle size, surface charge, PDI, SEM, MTT assay, and flow cytometry.
Main Results:
- Optimized nanoemulgel (AEE8) showed spherical shape, particle size of 226 nm, negative surface charge, and PDI of 0.157.
- AEE8 reduced A431 cell viability with an IC50 of 16.56 μg/mL.
- Flow cytometry revealed G2/M phase arrest, indicating cell cycle disruption.
Conclusions:
- The optimized nanoemulgel (AEE8) demonstrates enhanced anticancer efficacy compared to the extract alone.
- The nanoemulgel effectively inhibits epidermoid carcinoma cell proliferation by inducing cell cycle arrest.
- AEE8 is a promising, safe, and innovative candidate for anti-skin-cancer therapy.
