Related Experiment Video
Updated: Jul 21, 2025

Collection and Identification of Pollen from Honey Bee Colonies
Published on: January 19, 2021
Acceptance by Honey Bees of Wax Decontaminated through an Extraction Process with Methanol
José Manuel Flores1, Alba Luna2,3, Antonio Rodríguez Fernández-Alba4
1Department of Zoology, University of Córdoba, Campus of Rabanales, 14071 Córdoba, Spain.
Abstract:
Honey bees face serious threats. These include the presence of the Varroa destructor mite in hives, which requires the use of acaricides to control. The constant recycling of old wax exacerbates the problem, and results in the accumulation of residues in the beeswax, which is a problem for the viability of the colony. The same happens with the accumulation of phytosanitary residues. In a previous study, we implemented an efficient wax decontamination method using a batch methanol extraction method. The present study evaluates the acceptance of the decontaminated wax by the bees for comb building, brood, honey and pollen containment. The results show a slight delay in the start of comb building and small changes were observed in the pharmacopoeia of the decontaminated wax compared to the original commercial wax. The slight delay in the acceptance of the decontaminated wax could be due to the loss of some components, such as honey residues, which usually appear in the wax. The addition of bee-attractive substances to the manufacturing process could help to mitigate the delay. The results suggest that the use of decontaminated wax is a good alternative to reduce the concentration of residues in hives.
More Related Videos
06:41Preparation of Virus-Enriched Inoculum for Oral Infection of Honey Bees Apis mellifera
Published on: August 26, 2020
08:53Preparation of Single-cohort Colonies and Hormone Treatment of Worker Honeybees to Analyze Physiology Associated with Role and/or Endocrine System
Published on: September 6, 2016