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Marine-Derived Fungi as a Valuable Resource for Amylases Activity Screening
Di Zhang1, Lan Liu1,2, Bi-Shuang Chen1,2
1School of Marine Sciences, Sun Yat-Sen University, Zhuhai 519080, China.
Journal of Fungi (Basel, Switzerland)
|July 28, 2023
Summary
Marine fungi were screened for amylase production. Aspergillus flavus 9261 showed high amylase activity and stability, highlighting marine enzymes for biotechnology.
Area of Science:
- Marine microbiology
- Enzymology
- Biotechnology
Background:
- Marine microbial enzymes, including amylases, are crucial for industrial applications.
- Marine environments are a rich source of novel enzymes with unique properties.
Purpose of the Study:
- To screen marine-derived fungi for amylase-producing capabilities.
- To characterize the activity and stability of amylases from selected marine fungal isolates.
Main Methods:
- Isolation of fungi from South China Sea sediments.
- In vitro screening for amylase activity using the dinitrosalicylic acid (DNS) method.
- Determination of optimal pH and temperature, and assessment of thermal and pH stability.
Main Results:
- 57 fungal isolates exhibited amylase-producing activity.
- Aspergillus flavus 9261 demonstrated the highest amylase activity (10.7482 U/mg).
- The enzyme from Aspergillus flavus 9261 showed excellent thermostability and pH stability.
Conclusions:
- Marine fungi, particularly Aspergillus flavus 9261, are promising sources of industrially relevant amylases.
- The characterized marine amylase exhibits significant stability, making it valuable for biotechnological applications.
