Ion-to-Image, i2i, a Mass Spectrometry Imaging Data Analysis Platform for Continuous Ionization Techniques.
Johan Lillja1, Kyle D Duncan1,2, Ingela Lanekoff1
1Department of Chemistry - BMC, Uppsala University, Uppsala, 752 37, Sweden.
Analytical Chemistry
|July 28, 2023
Summary
A new MATLAB application, ion-to-image (i2i), simplifies processing of mass spectrometry imaging (MSI) data from continuous ionization sources. This tool handles complex datasets, enabling faster and more flexible ion image generation for researchers.
Area of Science:
- Analytical Chemistry
- Biotechnology
- Computational Chemistry
Background:
- Mass spectrometry imaging (MSI) provides high-sensitivity, selective molecular distribution data.
- Processing MSI data, especially from continuous ionization sources with automatic gain control (AGC), presents significant challenges.
- Existing software often lacks specialized handling for continuous ionization MSI data, necessitating new solutions.
Purpose of the Study:
- To introduce ion-to-image (i2i), a novel MATLAB-based application for processing MSI data.
- To address the specific data handling needs of continuous ionization sources, AGC, and high-resolution datasets.
- To offer a flexible and efficient tool for generating, processing, and exporting ion images from complex MSI data.
Main Methods:
- Development of a MATLAB-based application named ion-to-image (i2i).
- Implementation of quantitative, targeted, and nontargeted data processing strategies.
- Support for complex scan functions, including MSⁿ and SIM data, alongside full scans.
Main Results:
- i2i enables rapid processing of large and complex MSI datasets, reducing processing time to minutes.
- The application facilitates the generation of ion images from MSI data acquired with continuous ionization sources and AGC.
- Provides flexibility in data processing and export options for various MSI applications.
Conclusions:
- i2i offers a valuable, flexible, and efficient solution for handling specialized MSI data.
- The developed software addresses a critical gap in MSI data processing tools.
- i2i is expected to become a significant asset in the MSI software landscape.
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