Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

64
The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
64
CRISPR and crRNAs02:53

CRISPR and crRNAs

17.1K
Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
17.1K
The Antiviral System of Bacteria and Archaea: CRISPR01:23

The Antiviral System of Bacteria and Archaea: CRISPR

59
CRISPR stands for Clustered Regularly Interspaced Short Palindromic Repeats is a adaptive immune system found in bacteria and archaea that protects against viral infections. This system enables prokaryotic cells to identify, remember, and neutralize foreign genetic elements, primarily bacteriophages, by storing fragments of the invader’s DNA as a genetic memory.The CRISPR immune response begins during an initial infection. Cas (CRISPR-associated) proteins play a central role in this...
59
Homologous Recombination02:31

Homologous Recombination

50.7K
The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
50.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Steady-State and Dynamic Behavior of Geometry-Tunable Microfluidic Passive Flow Regulators.

Small (Weinheim an der Bergstrasse, Germany)·2026
Same author

Programmable Double Peptide Nucleic Acid-Locked Nucleic Acid Molecular Switch Enables Extraction-Free Direct Zygosity Discrimination of Single Nucleotide Polymorphisms.

Analytical chemistry·2026
Same author

Geospatial Analysis of Abortion Access for U.S. Servicewomen After <i>Dobbs</i>.

O&G open·2026
Same author

Integrated Biodevice for Parallel Magnetic Isolation and Bioelectrocatalytic Detection of Circulating DNA and RNA Biomarkers in Non-Small Cell Lung Cancer Liquid Biopsy.

ACS sensors·2026
Same author

Exploiting Device Deformability for Fluid and Particle Manipulation.

Small (Weinheim an der Bergstrasse, Germany)·2026
Same author

Simultaneous method validation and surveillance of pyrrolizidine alkaloids and their N-oxides in some medicinal plants and herbal teas using QuEChERS-LC-MS/MS.

Talanta·2026

Related Experiment Video

Updated: Jul 21, 2025

Field-Deployable Candidatus Liberibacter asiaticus Detection Using Recombinase Polymerase Amplification Combined with CRISPR-Cas12a
09:03

Field-Deployable Candidatus Liberibacter asiaticus Detection Using Recombinase Polymerase Amplification Combined with CRISPR-Cas12a

Published on: December 23, 2022

2.7K

CRISPR/Cas-Based Diagnostics in Agricultural Applications.

Tanzena Tanny1,2, Mohamed Sallam1,2, Narshone Soda2

  • 1School of Environment and Science (ESC), Griffith University, Nathan, QLD 4111, Australia.

Journal of Agricultural and Food Chemistry
|July 28, 2023
PubMed
Summary

Clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein (Cas) systems offer rapid and precise diagnostics for agricultural pests and diseases. These advanced tools show great promise for field-level disease detection and crop management.

Keywords:
CRISPR-CasGMOfast-track breedingnucleic acid detectionpathogen diagnosisplant protectionpoint-of-care testingtraditional strategies

More Related Videos

Author Spotlight: Development of Simplified CRISPR-Based Tests for Rapid Detection of Infectious Diseases
10:16

Author Spotlight: Development of Simplified CRISPR-Based Tests for Rapid Detection of Infectious Diseases

Published on: August 16, 2024

1.3K
Rapid and Specific Detection of Acinetobacter baumannii Infections Using a Recombinase Polymerase Amplification/Cas12a-based System
07:59

Rapid and Specific Detection of Acinetobacter baumannii Infections Using a Recombinase Polymerase Amplification/Cas12a-based System

Published on: April 25, 2025

356

Related Experiment Videos

Last Updated: Jul 21, 2025

Field-Deployable Candidatus Liberibacter asiaticus Detection Using Recombinase Polymerase Amplification Combined with CRISPR-Cas12a
09:03

Field-Deployable Candidatus Liberibacter asiaticus Detection Using Recombinase Polymerase Amplification Combined with CRISPR-Cas12a

Published on: December 23, 2022

2.7K
Author Spotlight: Development of Simplified CRISPR-Based Tests for Rapid Detection of Infectious Diseases
10:16

Author Spotlight: Development of Simplified CRISPR-Based Tests for Rapid Detection of Infectious Diseases

Published on: August 16, 2024

1.3K
Rapid and Specific Detection of Acinetobacter baumannii Infections Using a Recombinase Polymerase Amplification/Cas12a-based System
07:59

Rapid and Specific Detection of Acinetobacter baumannii Infections Using a Recombinase Polymerase Amplification/Cas12a-based System

Published on: April 25, 2025

356

Area of Science:

  • Agricultural Science
  • Molecular Biology
  • Biotechnology

Background:

  • Agricultural production is significantly hampered by pests and pathogens.
  • Early and efficient diagnostic methods are essential for effective disease management in crops.
  • CRISPR/Cas systems have emerged as powerful tools for nucleic acid detection.

Purpose of the Study:

  • To review recent advancements in CRISPR/Cas-based diagnostic tools for agriculture.
  • To explore the applications of CRISPR/Cas systems in diagnosing plant pathogens and identifying genetically modified crops.
  • To discuss challenges, solutions, and future perspectives of CRISPR/Cas diagnostics in agriculture.

Main Methods:

  • Review of current literature on CRISPR/Cas systems in agricultural diagnostics.
  • Analysis of CRISPR/Cas system components (Cas endonuclease, guide RNA) for target nucleic acid recognition and cleavage.
  • Comparison of CRISPR/Cas diagnostics with traditional methods like RT-PCR, LAMP, and NGS.

Main Results:

  • CRISPR/Cas systems demonstrate high sensitivity, specificity, and rapid assay times for agricultural diagnostics.
  • These systems are effective for identifying plant pathogens and detecting genetically modified crops.
  • CRISPR/Cas diagnostics offer advantages in multiplexing capabilities and suitability for point-of-care field testing.

Conclusions:

  • CRISPR/Cas-based diagnostics represent a significant advancement over traditional methods in agriculture.
  • The technology holds substantial potential for improving crop health monitoring and management.
  • Addressing current challenges will further unlock the full potential of CRISPR/Cas systems in diverse agricultural applications.