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Updated: Jul 21, 2025

Evaluation of Antimicrobial Activities of Nanoparticles and Nanostructured Surfaces In Vitro
Published on: April 21, 2023
Differential Antimicrobial Effect of Three-Sized Biogenic Silver Nanoparticles as Broad-Spectrum Antibacterial Agents
Munirah F Aldayel1, Nermin El Semary1,2, David G Adams3
1Biological Sciences Department, College of Science, King Faisal University, Al-Ahsa 31982, Saudi Arabia.
Background:
Massive fruit losses are caused by microbial pathogens of unknown identities. Therefore, ecofriendly biocontrol measures are well sought after, and biogenic silver nanoparticles are plausible candidates. Here we investigate the antimicrobial effect of three different sized AgNPs samples on those pathogens.
Methodology:
Identities of three local pathogenic bacteria were investigated using molecular methods. Three different-sized samples of silver nanoparticles were bio-synthesized in the external solution of a cyanobacterial culture, characterized, and used in antimicrobial bioassay.
Results:
The pathogens were identified as Erwinia pyrifoliae, Staphylococcus warneri, and Xanthomonas citri. UV-vis. and FTIR spectroscopy confirmed the biosynthesis of AgNPs. and their three different sizes were confirmed using Scanning electron microscopy. Growth of bacterial pathogens was inhibited by all three samples of AgNPs, but the largest inhibition zone was for the smallest sized AgNPs against Staphylococcus warneri (1.7 cm).
Discussion:
The identity of the pathogens infecting different local fruits is reported for the first time. They belong to different bacterial lineages. The fact that biogenic AAgNPs were effective against all of them shows their broad-spectrum of antibacterial effect. Customized biosynthesis was successful in yielding different-sized AgNPs. The smaller the AgNPs, the stronger the antimicrobial impact.
Conclusion:
Local bacterial species infecting fruits are diverse. Customized biogenic AgNPs are effective broad-spectrum biocontrol agents against bacterial pathogens of local fruits and thereby help maintain food security and environmental sustainability.
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