Related Experiment Video

Updated: Jul 21, 2025

Gastric Mucosa Quantitative Polymerase Chain Reaction Analysis for Detecting Helicobacter pylori and Antibiotic Resistance
05:23

Gastric Mucosa Quantitative Polymerase Chain Reaction Analysis for Detecting Helicobacter pylori and Antibiotic Resistance

Published on: March 7, 2025

423

Whole Genome Sequencing for Studying Helicobacter pylori Antimicrobial Resistance

Belén Fernández-Caso1, Ana Miqueleiz2, Teresa Alarcón3

  • 1Department of Microbiology, Hospital Valle del Nalón, 33920 Langreo, Spain.

Antibiotics (Basel, Switzerland)
|July 29, 2023
PubMed

Insights

Antibiotic resistance in Helicobacter pylori is a growing problem. Whole genome sequencing (WGS) helps understand resistance mechanisms to improve patient treatment and guidelines.

Area of Science:

  • Microbiology
  • Genomics
  • Infectious Diseases

Background:

  • Antibiotic resistance (AMR) is a significant global health threat.
  • Helicobacter pylori infections are common, and AMR compromises treatment efficacy.
  • Genomic insights into AMR are crucial for effective therapeutic strategies.

Purpose of the Study:

  • To review the application and evolution of whole genome sequencing (WGS) for studying AMR in Helicobacter pylori.
  • To highlight the value of WGS in understanding genomic resistance mechanisms.

Main Methods:

  • Utilizing next-generation sequencing (NGS) technologies.
  • Focusing on whole genome sequencing (WGS) as a molecular tool.
  • Analyzing genomic determinants of resistance to key antibiotics.

Main Results:

  • WGS provides deep insights into the genetic basis of AMR in H. pylori.
  • Understanding resistance mechanisms aids in personalized treatment decisions.
  • WGS data can inform the optimization of treatment guidelines.

Conclusions:

  • Whole genome sequencing is a powerful tool for investigating H. pylori antibiotic resistance.
  • Genomic surveillance of AMR is essential for public health and clinical practice.
  • Continued development of WGS pipelines enhances our ability to combat AMR.
Keywords:
Helicobacter pyloriantibioticresistancewhole genome sequencing

More Related Videos

Author Spotlight: Detection and Treatment of Helicobacter pylori Infection06:40

Author Spotlight: Detection and Treatment of Helicobacter pylori Infection

Published on: July 28, 2023

3.3K
Detection of Helicobacter pylori Infection and Antibiotic Resistance via Stool Quantitative Polymerase Chain Reaction Analysis04:56

Detection of Helicobacter pylori Infection and Antibiotic Resistance via Stool Quantitative Polymerase Chain Reaction Analysis

Published on: May 16, 2025

411

Related Concept Videos

Treating Helicobacter pylori in Peptic Ulcers: Antimicrobial Therapy01:16

Treating Helicobacter pylori in Peptic Ulcers: Antimicrobial Therapy

Helicobacter pylori, a resilient gram-negative bacterium, can thrive in the stomach's harsh, acidic environment. Infection with H. pylori leads to a cascade of events within the stomach lining. One of the critical disruptions caused by this bacterium is the interference with somatostatin production, a hormone responsible for regulating acid secretion. This interference tips the balance, escalating acid secretion and diminishing bicarbonate levels. This imbalance compromises the defensive...
456
Modern Molecular Taxonomy01:29

Modern Molecular Taxonomy

Advancements in molecular biology have revolutionized the identification and characterization of bacteria, with multiple methods leveraging DNA sequencing for enhanced precision. As sequencing technologies improve and costs decline, these approaches are increasingly used in clinical, environmental, and evolutionary studies.Multilocus Sequence Typing (MLST) examines several housekeeping genes, essential chromosomal genes encoding cellular functions, to distinguish strains. Approximately...
52
Genomic DNA in Prokaryotes00:46

Genomic DNA in Prokaryotes

The genome of most prokaryotic organisms consists of double-stranded DNA organized into one circular chromosome in a region of cytoplasm called the nucleoid. The chromosome is tightly wound, or supercoiled, for efficient storage. Prokaryotes also contain other circular pieces of DNA called plasmids. These plasmids are smaller than the chromosome and often carry genes that confer adaptive functions, such as antibiotic resistance.
Genomic Diversity in Bacteria
Although bacterial genomes are much...
44.0K
Antibiotic Selection00:57

Antibiotic Selection

Overview
54.8K
Development of Antibiotic Resistance01:30

Development of Antibiotic Resistance

Antibiotic resistance is a major public health concern that arises when bacteria evolve mechanisms to withstand the effects of antibiotic treatments. This resistance can be intrinsic, acquired through genetic mutations, or transferred between bacteria via horizontal gene transfer. The development of antibiotic resistance poses significant challenges in treating bacterial infections and necessitates ongoing research to develop new therapeutic strategies.Intrinsic resistance occurs when bacterial...
41