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Related Concept Videos

The Spindle Assembly Checkpoint02:19

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The spindle assembly checkpoint is a molecular surveillance mechanism ensuring the fidelity of chromosome segregation during anaphase. The checkpoint monitors the completion of all the prerequisite steps before chromosome segregation to determine whether the segregation process should proceed or be delayed.
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Checkpoints throughout the cell cycle serve as safeguards and gatekeepers, allowing the cell cycle to progress in favorable conditions and slow or halt it in problematic ones. This regulation is known as the cell cycle control system.
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At the transition from prophase to metaphase, there is a reduction in cohesion along the chromosomal arms, resulting in the resolution of sister chromatids. However, residual cohesin connections remain to hold the sister chromatids together until the transition from metaphase to anaphase. The residual connection prevents any premature separation of sister chromatids, blocking the risks of aneuploidy within the daughter cells.
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The Mitotic Spindle02:27

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The cell cycle regulation directs how a cell proceeds from one phase to the next and begins mitosis. The cell cycle control system includes intracellular regulatory molecules and external triggers. They provide "stop" or "advance" signals and operate at specific cell cycle stages termed checkpoints to ensure that a particular process is completed before the cell advances to the next phase.
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Related Experiment Video

Updated: Jul 21, 2025

Use of Time-Lapse Microscopy and Stage-Specific Nuclear Depletion of Proteins to Study Meiosis in S. cerevisiae
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Spindle Position Checkpoint Kinase Kin4 Regulates Organelle Transport in Saccharomyces cerevisiae.

Lakhan Ekal1, Abdulaziz M S Alqahtani1,2, Maya Schuldiner3

  • 1School of Biosciences, University of Sheffield, Sheffield S10 2TN, UK.

Biomolecules
|July 29, 2023
PubMed
Summary

The spindle position checkpoint kinase Kin4 regulates peroxisome transport in yeast, independent of its known role. Kin4 and Frk1 protect the peroxisomal Myo2 receptor, impacting organelle inheritance.

Keywords:
actin cytoskeletonclass V myosinmitotic exit network (MEN)organelle transportperoxisomespindle position checkpoint (SPOC)

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Area of Science:

  • Cell Biology
  • Molecular Biology
  • Genetics

Background:

  • Membrane-bound organelles are crucial for eukaryotic cellular metabolism and require monitoring for proper function.
  • The actin cytoskeleton and actomyosin-based motor proteins are essential for organelle transport and inheritance in eukaryotes.
  • While class V Myosins are known factors in yeast organelle inheritance, the regulation of this transport is not fully understood.

Purpose of the Study:

  • To investigate the spatiotemporal regulation of actomyosin-based organelle transport in yeast.
  • To identify novel regulators of peroxisome inheritance using a genome-wide screen.

Main Methods:

  • An automated genome-wide genetic screen in *Saccharomyces cerevisiae* (yeast).
  • Utilized peroxisome inheritance as a model system to study organelle transport.
  • Investigated the role of the spindle position checkpoint (SPOC) kinase Kin4 and its paralog Frk1.

Main Results:

  • The SPOC kinase Kin4, and to a lesser extent Frk1, regulate peroxisome transport independently of their SPOC function.
  • Kin4's kinase activity is necessary for its role in regulating peroxisome transport.
  • Kin4 and Frk1 protect Inp2, the peroxisomal Myo2 receptor, from degradation, thereby influencing organelle inheritance.
  • Vacuole inheritance is also affected in cells deficient in Kin4/Frk1, suggesting a shared regulatory mechanism.

Conclusions:

  • Kin4 and Frk1 are novel regulators of actomyosin-based organelle transport in yeast, impacting peroxisome and vacuole inheritance.
  • These findings reveal a common regulatory mechanism for actin-based transport of different organelles.
  • The study provides new insights into the spatiotemporal control of organelle dynamics and maintenance in eukaryotic cells.