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Related Concept Videos

Epistasis01:39

Epistasis

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In addition to multiple alleles at the same locus influencing traits, numerous genes or alleles at different locations may interact and influence phenotypes in a phenomenon called epistasis. For example, rabbit fur can be black or brown depending on whether the animal is homozygous dominant or heterozygous at a TYRP1 locus. However, if the rabbit is also homozygous recessive at a locus on the tyrosinase gene (TYR), it will have an unshaded coat that appears white, regardless of its TYRP1...
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Related Experiment Video

Updated: Jul 21, 2025

Development of an in vitro model system for studying the interaction of Equus caballus IgE with its high-affinity receptor Fc&#949;RI
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High-Resolution Genotyping of Expressed Equine MHC Reveals a Highly Complex MHC Structure.

Deepali Vasoya1, Thomas Tzelos1,2, Lindert Benedictus3

  • 1The Roslin Institute, The Royal (Dick) School of Veterinary Studies, University of Edinburgh, Easter Bush Campus, Roslin EH25 9RG, UK.

Genes
|July 29, 2023
PubMed
Summary
This summary is machine-generated.

High-throughput sequencing revealed extensive Major Histocompatibility Complex (MHC) diversity in horses. This study characterized novel equine MHCI and MHCII sequences, highlighting breed-specific variations and broader population diversity.

Keywords:
Major Histocompatibility Complex (MHC)breed diversityequinehigh-throughput sequencingpolymorphism

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Area of Science:

  • Immunogenetics
  • Comparative Genomics
  • Equine Science

Background:

  • The Major Histocompatibility Complex (MHC) genes are crucial for immune responses and are highly polymorphic.
  • Previous characterization of equine MHC genes was limited by technology, especially in non-model breeds.
  • High-throughput sequencing offers advanced methods for analyzing complex gene families like MHC.

Purpose of the Study:

  • To develop and apply a MiSeq-based approach for analyzing expressed MHCI and MHCII repertoires in horses.
  • To generate high-resolution data on the equine MHC.
  • To investigate MHC diversity across different horse breeds.

Main Methods:

  • Utilized a MiSeq-based high-throughput sequencing approach.
  • Analyzed combined expressed MHCI and MHCII repertoires.
  • Studied cohorts of Thoroughbred, Icelandic, and Norwegian Fjord Horses (n=168).

Main Results:

  • Generated comprehensive MHCI/II data for all 168 horses.
  • Identified 152 novel equine MHCI sequences and 117 novel MHCII sequences.
  • Observed limited MHC haplotype overlap between Thoroughbreds and Icelandic/Norwegian Fjord Horses, indicating significant breed-specific variation.

Conclusions:

  • The study provides novel insights into the expressed equine MHC repertoire structure.
  • Significant MHC diversity exists within and between horse breeds.
  • Further exploration of global horse populations is expected to reveal even greater MHC diversity.