Related Experiment Video

Updated: Jul 21, 2025

Optimization of a Multiplex RNA-based Expression Assay Using Breast Cancer Archival Material
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Published on: August 1, 2018

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Loss of ANCO1 Expression Regulates Chromatin Accessibility and Drives Progression of Early-Stage Triple-Negative

Meng Yuan1, Megan E Barefoot1, Kendell Peterson1

  • 1Lombardi Comprehensive Cancer Center, Georgetown University, Washington, DC 20057, USA.

International Journal of Molecular Sciences
|July 29, 2023
PubMed

Insights

Loss of ANCO1 (ankyrin repeat domain containing 11) expression indicates poor breast cancer prognosis. Its depletion promotes invasion and activates oncogenic pathways, suggesting ANCO1 acts as a tumor suppressor.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Mutations in ANKRD11 (ANCO1) are linked to neurodegenerative disorders.
  • Loss of heterozygosity and low ANCO1 expression are observed in certain cancers.

Purpose of the Study:

  • To investigate the role of ANCO1 in breast cancer progression.
  • To determine if ANCO1 expression levels are prognostic markers for breast cancer outcomes.

Main Methods:

  • Analysis of ANCO1 mRNA and protein expression in breast cancer patient samples.
  • ANCO1 knockdown in triple-negative breast cancer (TNBC) cells and assessment of cellular phenotypes (aneuploidy, senescence, invasion).
  • ChIP-seq analysis to identify regulatory elements and associated transcription factors affected by ANCO1 depletion.

Main Results:

  • Low ANCO1 expression is a prognostic marker for poor clinical outcomes in breast cancer, particularly for overall survival in TNBC.
  • ANCO1 knockdown induced aneuploidy, senescence, and enhanced invasion in TNBC cells.
  • ANCO1 depletion globally increased H3K27Ac signals, enriched for AP-1, TEAD, STAT3, and NFκB motifs, and activated genes in PI3K-AKT, EMT, and senescence pathways.

Conclusions:

  • ANCO1 functions as a tumor suppressor in breast cancer.
  • Loss of ANCO1 expression activates breast cancer-specific enhancers and oncogenic pathways, accelerating early-stage tumor progression.
Keywords:
ANCO1ANKRD11TNBCbreast cancer enhancertumor suppressor

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