Related Experiment Video

Updated: Jul 21, 2025

Comparing Metastatic Clear Cell Renal Cell Carcinoma Model Established in Mouse Kidney and on Chicken Chorioallantoic Membrane
05:36

Comparing Metastatic Clear Cell Renal Cell Carcinoma Model Established in Mouse Kidney and on Chicken Chorioallantoic Membrane

Published on: February 8, 2020

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NCOA7 Regulates Growth and Metastasis of Clear Cell Renal Cell Carcinoma via MAPK/ERK Signaling Pathway

Jiayu Guo1,2, Shuai Ke2, Qi Chen1,2

  • 1Department of Organ Transplantation, Renmin Hospital of Wuhan University, Wuhan 430060, China.

International Journal of Molecular Sciences
|July 29, 2023
PubMed

Insights

Nuclear receptor coactivator NCOA7 is downregulated in clear cell renal cell carcinoma (ccRCC). NCOA7 overexpression inhibits ccRCC progression by affecting the MAPK/ERK pathway, suggesting its potential as a prognostic marker and therapeutic target.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Nuclear receptor coactivator NCOA7 is often downregulated in various cancers.
  • The role and prognostic value of NCOA7 in clear cell renal cell carcinoma (ccRCC) are currently unknown.

Purpose of the Study:

  • To investigate the expression and prognostic significance of NCOA7 in ccRCC.
  • To elucidate the functional role and molecular mechanisms of NCOA7 in ccRCC progression.

Main Methods:

  • Bioinformatics analysis, Western blotting, and immunohistochemistry were used to analyze NCOA7 expression in ccRCC tissues.
  • Kaplan-Meier analysis, ROC curve analysis, and clinicopathological correlation were employed to assess prognostic value.
  • In vitro and in vivo overexpression experiments were conducted in ccRCC cell lines and mouse models.

Main Results:

  • NCOA7 expression was found to be downregulated across all three subtypes of renal cell carcinoma, with significant prognostic value specifically for ccRCC patients.
  • Overexpression of NCOA7 suppressed the proliferation, invasion, and metastasis of ccRCC cells.
  • NCOA7 was found to inhibit the MAPK/ERK pathway, regulating epithelial-mesenchymal transformation (EMT) and apoptosis.

Conclusions:

  • NCOA7 acts as a tumor suppressor in ccRCC by inhibiting tumor growth and metastasis via the MAPK/ERK pathway.
  • NCOA7 demonstrates potential as a valuable prognostic biomarker and a therapeutic target for ccRCC treatment.
Keywords:
MAPK pathwayNCOA7clear cell renal cell carcinomaepithelial–mesenchymal transformation (EMT)molecular target

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