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Updated: Jul 21, 2025

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Comparison of Compound Identification Tools Using Data Dependent and Data Independent High-Resolution Mass

Rosalie Nijssen1, Marco H Blokland1, Robin S Wegh1

  • 1Wageningen Food Safety Research, Part of Wageningen University and Research, Akkermaalsbos 2, 6708 WB Wageningen, The Netherlands.

Metabolites
|July 29, 2023
PubMed
Summary

Evaluating four high-resolution mass spectrometry (HRMS) identification tools for pesticide and drug metabolite analysis revealed that mzCloud performed best for data-dependent acquisition (DDA) spectra. MSfinder showed the highest success rate for data-independent acquisition (DIA) spectra, though overall identification rates were lower for DIA.

Keywords:
annotationdata dependent acquisitiondata independent acquisitionhigh-resolution mass spectrometryidentificationmetabolitespesticidesveterinary drugs

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