A Modified-Herringbone Micromixer for Assessing Zebrafish Sperm (MAGS)

Area of Science:

• Aquatic biology
• Reproductive science
• Microfluidics

Background:

• Sperm motility analysis in aquatic species is crucial but faces challenges like small sample volumes and inconsistent activation.
• Existing microfluidic mixers improve reproducibility but require optimization for ease of use and wider adoption.

Purpose of the Study:

• To develop and optimize a novel microfluidic device for standardized and efficient activation of aquatic sperm.
• To ensure compatibility with standard computer-assisted sperm analysis (CASA) systems.

Main Methods:

• A novel micromixer geometry with modified herringbone structures was designed for rapid, consistent sperm activation via a 1:6 dilution with water.
• The polydimethylsiloxane (PDMS) chip supports both positive and negative pressure configurations for sample handling with a micropipettor.
• The device was optimized for zebrafish sperm activation and validated for use with CASA systems.

Main Results:

• The microfluidic device achieved rapid and consistent initiation of sperm motility.
• The device demonstrated practical utility with standard computer-assisted sperm analysis (CASA) systems.
• The design is adaptable for various aquatic species and can be implemented using 3D resin printing for open hardware accessibility.

Conclusions:

• The developed microfluidic device offers a standardized and simplified method for aquatic sperm activation, enhancing reproducibility.
• The adaptable design and open hardware potential promote wider adoption and collaboration in aquatic reproductive research.