Pathogenicity of Trichoderma afroharzianum in Cereal Crops

Species of the genus Trichoderma occur ubiquitously in soils, on plant roots and in decaying plant residues. Due to its competitiveness and mycoparasitic potential against other microorganisms, particular strains of Trichoderma spp. are used in agriculture as biocontrol agents against plant pathogens. However, Trichoderma afroharzianum has been recently reported as a pathogen causing ear rot disease on maize in Germany, France and Italy, leading to massive infections on maize cobs. This raised the question, whether and to what extent Trichoderma spp. can infect cereal crops other than maize and cause disease symptoms and yield losses. To address this question, two varieties of wheat, barley and sorghum were grown in the greenhouse and artificially inoculated with T. afroharzianum by both spray and point inoculation at the time of flowering. Disease severity was scored weekly, and thousand-kernel weight and colonization rate were determined after harvest. As early as 14 days after inoculation, the first visual symptoms appeared on wheat and barley as tan or brown discoloration of the base of a floret within the spikelets. After spray inoculation, clear discolorations of the entire ear were seen, while point inoculation only showed symptoms at the injection site and above. No visible symptoms were observed on sorghum millet. The colonization rate on wheat and barley grains was significantly increased compared to the control, while thousand-kernel weights (TKWs) were significantly reduced. No differences in colonization rate and TKW compared to the control were observed in sorghum. This is the first report of Trichoderma afroharzianum infecting wheat and barley, causing disease symptoms and significantly reducing thousand-kernel weights.