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Purification of Active Photosystem I-Light Harvesting Complex I from Plant Tissues
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Solarplast®-An Enzymatically Treated Spinach Extract.

Annie Simon1, Shahneela Mazhar1, Ekaterina Khokhlova1

  • 1ADM Cork H&W Limited, Food Science Building, University College Cork, T12 Y337 Cork, Ireland.

Plants (Basel, Switzerland)
|July 29, 2023
PubMed
Summary

Solarplast®, a spinach-derived supplement, shows enhanced antioxidant activity and protects cells from oxidative stress compared to regular spinach. This plant-based food supplement may help reduce oxidative stress in the human body.

Keywords:
HT-29HepG2Solarplast®antioxidantoxidative stressspinach

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Area of Science:

  • Biochemistry
  • Nutrition Science
  • Cell Biology

Background:

  • Environmental stimuli cause oxidative stress, necessitating antioxidant defense.
  • Dietary antioxidants, particularly from leafy greens like spinach, are crucial.
  • Limited bioavailability of spinach antioxidants due to indigestible cell walls.

Purpose of the Study:

  • To evaluate the antioxidant potential and enzymatic capabilities of Solarplast®.
  • To compare Solarplast® with frozen spinach regarding antioxidant activity.
  • To assess Solarplast®'s efficacy in mitigating cellular oxidative stress.

Main Methods:

  • Utilized analytical techniques and in vitro bioassays (TOC, FRAP, TEAC).
  • Quantified antioxidant enzymes in Solarplast® and frozen spinach.
  • Assessed cellular protection against oxidative stress in intestinal (HT-29) and liver (HepG2) cell lines.

Main Results:

  • Solarplast® exhibited superior antioxidant activity compared to frozen spinach.
  • Increased levels of several antioxidant enzymes were observed in Solarplast®.
  • Solarplast® effectively reduced oxidative stress and cytotoxicity in both cell lines.

Conclusions:

  • Solarplast® demonstrates enhanced antioxidant properties and cellular protective effects.
  • The cell-wall-free nature of Solarplast® may improve antioxidant bioavailability.
  • Solarplast® shows potential as a non-GMO, plant-based supplement for managing oxidative stress.