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Experimental Infection with Listeria monocytogenes as a Model for Studying Host Interferon-γ Responses
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Experimental Infection with Listeria monocytogenes as a Model for Studying Host Interferon-γ Responses

Published on: November 16, 2016

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Membrane vesicles derived from Listeria monocytogenes might be a potential antigen delivery vector

Mingyuan Tang1, Sicheng Tian1, Kehan Chen1

  • 1Department of Public Health Laboratory Sciences, West China School of Public Health and West China Fourth Hospital, Sichuan University, Chengdu 610061, China.

International Journal of Pharmaceutics
|July 29, 2023
PubMed

Insights

Listeria monocytogenes membrane vesicles (MVs) are safe and immunogenic, acting as effective humoral immune adjuvants. These MVs show potential as novel delivery vectors for antigens and drugs.

Area of Science:

  • Bacteriology
  • Immunology
  • Nanotechnology

Background:

  • Membrane vesicles (MVs) from Listeria monocytogenes (LM) are nanoscale and contain bacterial components.
  • LM MVs are being investigated as potential novel delivery vectors.
  • Safety and immunogenicity of LM MVs require in vivo evaluation.

Purpose of the Study:

  • To assess the safety and immunogenicity of LM MVs in vitro and in vivo.
  • To determine the potential of LM MVs as antigen and drug delivery vectors.
  • To evaluate the adjuvant properties of LM MVs.

Main Methods:

  • Isolation of LM MVs.
  • In vitro stimulation of RAW264.7 and DC2.4 cells with LM MVs.
  • In vivo safety assessment via intraperitoneal injection in C57BL/6 mice.
  • In vivo immunogenicity testing through intraperitoneal immunization and subcutaneous co-injection with ovalbumin.

Main Results:

  • LM MVs induced secretion of inflammatory cytokines (IL-1β, TNF-α, IL-6, IL-10) in immune cells.
  • Intraperitoneal injection of LM MVs showed no lethal effects or organ damage in mice, confirming safety.
  • LM MVs induced high levels of LM MV-specific IgG antibodies after immunization.
  • LM MVs demonstrated humoral immune adjuvant effects comparable to alum when co-injected with ovalbumin.

Conclusions:

  • LM MVs possess good safety and effective immunogenicity.
  • LM MVs can function as humoral immune adjuvants.
  • LM MVs represent a promising new option for antigen and drug delivery vectors.
Keywords:
ImmunogenicityListeria monocytogenesMembrane vesiclesSafety

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