Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

RNA-seq03:21

RNA-seq

10.1K
RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
10.1K
Next-generation Sequencing03:00

Next-generation Sequencing

91.5K
The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features....
91.5K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Hydropeaking strands and displaces larval and juvenile fish across species.

Communications earth & environment·2026
Same author

Novel technique for correcting urovagina via urethral transposition in the horse.

Equine veterinary journal·2026
Same author

Rare twin cysteine residues in the HIV-1 envelope variable region 1 link to neutralization escape and breadth development.

Cell host & microbe·2026
Same author

How Vaccinating People Living With HIV May Guide bNAb‑Based Vaccines.

Journal of the International AIDS Society·2026
Same author

Progressive bilateral dorsal swelling of the neck from the level of the occiput to the level of C3 in a 17-year-old warmblood gelding.

Journal of the American Veterinary Medical Association·2026
Same author

Variants in human CD48 lead to impaired T cell immunity and increased inflammation.

The Journal of clinical investigation·2026

Related Experiment Video

Updated: Jul 20, 2025

Author Spotlight: A Cost-Effective Genomic Workflow for Advancing Rabies Control in Resource-Limited Settings
10:26

Author Spotlight: A Cost-Effective Genomic Workflow for Advancing Rabies Control in Resource-Limited Settings

Published on: August 18, 2023

5.3K

Rapid and sensitive single-sample viral metagenomics using Nanopore Flongle sequencing.

Ian Pichler1, Stefan Schmutz1, Gabriela Ziltener1

  • 1Institute of Medical Virology, University of Zurich, Winterthurerstrasse 190, 8057 Zurich, Switzerland.

Journal of Virological Methods
|July 29, 2023
PubMed
Summary

This study introduces a faster, cheaper viral metagenomic Next-Generation Sequencing (mNGS) method using Nanopore Flongle technology. This approach enables rapid and cost-effective viral detection in clinical diagnostics, improving turnaround times for patient care.

Keywords:
Clinical diagnosticsFlongle flow cellsNanopore sequencingViral metagenomics

More Related Videos

Nanopore DNA Sequencing for Metagenomic Soil Analysis
07:33

Nanopore DNA Sequencing for Metagenomic Soil Analysis

Published on: December 14, 2017

30.6K
Sequencing of mRNA from Whole Blood using Nanopore Sequencing
11:26

Sequencing of mRNA from Whole Blood using Nanopore Sequencing

Published on: June 3, 2019

13.7K

Related Experiment Videos

Last Updated: Jul 20, 2025

Author Spotlight: A Cost-Effective Genomic Workflow for Advancing Rabies Control in Resource-Limited Settings
10:26

Author Spotlight: A Cost-Effective Genomic Workflow for Advancing Rabies Control in Resource-Limited Settings

Published on: August 18, 2023

5.3K
Nanopore DNA Sequencing for Metagenomic Soil Analysis
07:33

Nanopore DNA Sequencing for Metagenomic Soil Analysis

Published on: December 14, 2017

30.6K
Sequencing of mRNA from Whole Blood using Nanopore Sequencing
11:26

Sequencing of mRNA from Whole Blood using Nanopore Sequencing

Published on: June 3, 2019

13.7K

Area of Science:

  • Clinical diagnostics
  • Virology
  • Genomics

Background:

  • Viral metagenomic Next-Generation Sequencing (mNGS) offers unbiased detection of viral nucleic acids for diagnosing complex infections.
  • Current mNGS diagnostics often rely on Illumina sequencing, which is accurate but limited by long turnaround times and high costs.
  • This hinders the widespread adoption of mNGS in routine clinical practice.

Purpose of the Study:

  • To develop and evaluate a rapid, cost-effective viral mNGS protocol for clinical diagnostics.
  • To reduce sample-to-result times compared to existing Illumina-based mNGS methods.
  • To assess the sensitivity and accuracy of the new protocol for detecting DNA/RNA viruses.

Main Methods:

  • Development of a novel mNGS protocol utilizing Nanopore Flongle sequencing.
  • Optimization of sample preparation and sequencing steps to decrease overall time.
  • Direct comparison of Nanopore Flongle mNGS with Illumina mNGS using diverse clinical samples.

Main Results:

  • The Nanopore Flongle protocol significantly reduced sample preparation (6 hours) and sequencing (2 hours) times.
  • The method demonstrated sensitive detection of DNA/RNA viruses, even at low input levels (up to 33-38 Ct).
  • Comparative analysis showed comparable diagnostic results between Nanopore Flongle and Illumina mNGS.

Conclusions:

  • The developed Nanopore Flongle mNGS protocol provides a rapid and cost-effective alternative for clinical viral diagnostics.
  • This approach is suitable for individual testing of severe cases, addressing the limitations of current methods.
  • The study highlights the potential of Nanopore sequencing for improving turnaround times and accessibility in viral diagnostics.