Non-invasive Microneedle Application Increases Ceramide and Natural Moisturizing Factors in a Reconstructed Human...

Area of Science:

• Dermatology
• Cosmetic Science
• Biotechnology

Background:

• Microneedling is a popular cosmetic treatment for improving skin barrier and moisturizing functions, potentially reducing wrinkles.
• Reported side effects like erythema and edema necessitate the development of safer microneedle cosmetic alternatives.

Purpose of the Study:

• To investigate the efficacy of non-invasive microneedle application in enhancing skin barrier and moisturizing functions.
• To determine if microneedles can improve skin health without penetrating the stratum corneum, thereby minimizing side effects.

Main Methods:

• Established conditions for non-penetrating microneedle application on reconstructed human full-thickness skin models.
• Analyzed gene expression related to skin barrier components (SPTLC3, filaggrin, transglutaminase 1) and degradation products.
• Quantified ceramide production and free amino acid levels post-microneedling.

Main Results:

• Non-invasive microneedle application significantly increased gene expression of serine palmitoyltransferase long chain base subunit (SPTLC) 3, filaggrin, and transglutaminase 1.
• Increased ceramide production, a key component of the skin barrier, was observed.
• Elevated gene expression of filaggrin-degrading enzymes and increased levels of free amino acids (natural moisturizing factors) were noted.

Conclusions:

• Non-invasive microneedle application effectively enhances skin barrier and moisturizing functions.
• This method increases ceramide and natural moisturizing factor production, suggesting a safer approach to microneedle cosmetics.
• The findings support the potential of non-penetrating microneedles for cosmetic applications aimed at skin health improvement.