Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Regulated Protein Degradation

Regulated Protein Degradation

The Proteasome

The Proteasome

Eukaryotic cells can degrade proteins through several pathways. One of the most important amongst these is the ubiquitin-proteasome pathway. It helps the cell eliminate the misfolded, damaged, or unwarranted cytoplasmic proteins in a highly specific manner.
In this pathway, the target proteins are first tagged with small proteins called ubiquitin. A series of enzymes carry out the ubiquitination of the target proteins - E1 (ubiquitin-activating enzyme), E2 (ubiquitin-conjugating enzyme), and E3...

The Proteasome Structure

The Proteasome Structure

The ubiquitin-proteasome pathway is a well-known mechanism utilized by eukaryotic cells to remove cytoplasmic proteins that are misfolded, damaged, or no longer needed. In this pathway, the protein that needs to be eliminated undergoes a process called ubiquitination, where a chain of ubiquitin molecules is attached to the 48th lysine residue of the target protein. This ubiquitin modification helps the proteasome distinguish between a target protein and a healthy protein.
The proteasome is an...

Proteins: From Genes to Degradation

Proteins: From Genes to Degradation

Directing Proteins to the Rough Endoplasmic Reticulum

Directing Proteins to the Rough Endoplasmic Reticulum

The organelle-specific signaling sequences direct proteins synthesized in the cytosol to their final destination like ER, mitochondria, peroxisomes, etc. Some of the proteins directed to ER are then trafficked via vesicles to other organelles within the cell or the extracellular environment through the Golgi complex. For example, the rough ER synthesizes soluble proteins for transportation to the lysosomes or secretion out of the cell. It can also synthesize transmembrane proteins that can...

Protein Networks

Protein Networks

An organism can have thousands of different proteins, and these proteins must cooperate to ensure the health of an organism. Proteins bind to other proteins and form complexes to carry out their functions. Many proteins interact with multiple other proteins creating a complex network of protein interactions.
These interactions can be represented through maps depicting protein-protein interaction networks, represented as nodes and edges. Nodes are circles that are representative of a protein,...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Senescent Cell Derived Artificial Vesicle-Based Senolytic Sonovaccine Platform with Augmented Lymph Node Delivery and Antigen Cross-Presentation Efficacy.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2026

Same author

Potential Strategies for Bacterial Infection Control: Outer Membrane Vesicle Based Bacterial Vaccines.

Advanced healthcare materials·2026

Same author

Hybrid macrophage-mitochondria extracellular vesicles for mitochondrial ROS regulation in diabetic wounds.

Nature communications·2026

Same author

Deracemization of C(sp<sup>3</sup>)-H Arylated Carbonyl Compounds via Asymmetric Ion-Pairing Photoredox Catalysis.

Journal of the American Chemical Society·2025

Same author

Toward Ultrasound Molecular Imaging of Endothelial Dysfunction in Diabetes: Targets, Strategies, and Challenges.

ACS applied bio materials·2024

Same author

Oxidative Dehydrogenation of Alkanes through Homogeneous Base Metal Catalysis.

Chemical record (New York, N.Y.)·2023

Same journal

MRC2 Links Collagen Turnover to Integrin/PI3K/AKT-Driven Fibrogenesis.

Advanced biology·2026

Same journal

Integrative Database-Driven In Silico and In Vitro Study of Anemarrhena asphodeloides Bunge Highlighting Hippeastrine as a Regulator of the HSP90/PI3K/Akt/mTOR Axis in Oral Squamous Cell Carcinoma.

Advanced biology·2026

Same journal

miR-210 in Ischemic Cardiovascular Disease: Functions, Mechanisms, and Therapeutic Potential.

Advanced biology·2026

Same journal

From Spider Webs to Brains: Can the Bri2 BRICHOS Molecular Chaperone Domain Transport Biological Drugs Into the Central Nervous System?

Advanced biology·2026

Same journal

RNAi-Loaded Exosomes for the Management of Breast Cancer: Emerging Paradigms in Precision Therapy.

Advanced biology·2026

Same journal

Cross-Species Transmission and Structural Adaptive Evolution: Implications of the FCoV-23 Pandemic for the Evolution of Coronaviruses.

Advanced biology·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Jul 20, 2025

High-Throughput Cellular Profiling of Targeted Protein Degradation Compounds Using HiBiT CRISPR Cell Lines

High-Throughput Cellular Profiling of Targeted Protein Degradation Compounds Using HiBiT CRISPR Cell Lines

Published on: November 9, 2020

Targeted Protein Degradation: Principles, Strategies, and Applications.

Ting He¹, Chenxi Wen¹, Guodong Yang²

¹Department of Burns and Cutaneous Surgery, Xijing Hospital, Fourth Military Medical University, Xi'an, 710032, P. R. China.

Advanced Biology

|July 30, 2023

Summary

This summary is machine-generated.

Targeted protein degradation leverages cellular machinery to eliminate specific proteins. This review explores recent advancements, applications, and challenges in this potent biological research and drug discovery tool.

Keywords:

autophagy-lysosome pathway clinical application endosome-lysosome system targeted protein degradation ubiquitin-proteasome system

More Related Videos

Author Spotlight: Evaluating Biophysical Assays for Characterizing PROTACS Ternary Complexes

Author Spotlight: Evaluating Biophysical Assays for Characterizing PROTACS Ternary Complexes

Published on: January 12, 2024

Tuning Degradation to Achieve Specific and Efficient Protein Depletion

Tuning Degradation to Achieve Specific and Efficient Protein Depletion

Published on: July 20, 2019

Related Experiment Videos

Last Updated: Jul 20, 2025

High-Throughput Cellular Profiling of Targeted Protein Degradation Compounds Using HiBiT CRISPR Cell Lines

High-Throughput Cellular Profiling of Targeted Protein Degradation Compounds Using HiBiT CRISPR Cell Lines

Published on: November 9, 2020

Author Spotlight: Evaluating Biophysical Assays for Characterizing PROTACS Ternary Complexes

Author Spotlight: Evaluating Biophysical Assays for Characterizing PROTACS Ternary Complexes

Published on: January 12, 2024

Tuning Degradation to Achieve Specific and Efficient Protein Depletion

Tuning Degradation to Achieve Specific and Efficient Protein Depletion

Published on: July 20, 2019

Area of Science:

Cell Biology
Molecular Biology
Biochemistry

Background:

Cellular homeostasis relies on regulated protein degradation.
Intracellular protein quality control involves the ubiquitin-proteasome system and lysosome pathway.
Physiological protein degradation inspires novel degradation strategies.

Purpose of the Study:

To review recent advances in targeted protein degradation techniques.
To summarize the principles, advantages, and challenges of these methods.
To discuss potential applications in biological science and clinical settings.

Main Methods:

Literature review of targeted protein degradation strategies.
Analysis of current techniques and their underlying mechanisms.
Exploration of emerging applications and future research directions.

Main Results:

Targeted protein degradation offers potent tools for research and drug discovery.
Various techniques have been developed based on cellular degradation pathways.
Significant progress has been made in understanding and applying these methods.

Conclusions:

Targeted protein degradation techniques are powerful tools in modern biology and medicine.
Continued research promises expanded applications in disease treatment and biological studies.
Addressing current challenges will further enhance the utility of these degradation strategies.