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Expression profiles of MMP-9 and EMMPRIN in chronic rhinosinusitis with nasal polyps
Spyridon Lygeros1, Gerasimos Danielides1, George C Kyriakopoulos2
1Department of Otorhinolaryngology, School of Medicine, University of Patras, Patras, Greece.
Objective:
Metalloproteinases (MMPs) are implicated in tissue remodeling in chronic rhinosinusitis with nasal polyps (CRSwNP). This study aimed to evaluate the expression profiles of MMP-9 and the extracellular matrix metalloproteinase inducer (EMMPRIN) in nasal polyps compared to healthy mucosa.
Methods:
Tissue samples from 37 CRSwNP patients undergoing functional endoscopic sinus surgery and mucosal specimens from 12 healthy controls were obtained intra-operatively. MMP-9 and EMMPRIN mRNA levels were assessed by reverse transcription-polymerase chain reaction and their protein expression by Western blot analysis.
Results:
MMP-9 mRNA expression levels were significantly elevated in CRSwNP compared to controls (p < 0.05). MMP-9 protein levels presented an increasing trend but with no statistical significance (p > 0.05). No statistically significant difference in EMMPRIN mRNA and protein levels was identified.
Conclusions:
Upregulation of MMP-9 in nasal polyps is evident and highlights its role in the pathogenesis of CRSwNP. The lack of concordance between MMP-9 mRNA and protein levels may be attributed to post-translational gene expression regulation. Although EMMPRIN expression was not significantly different between the two groups, its role remains to be elucidated. MMP-9 may be a valuable biomarker and treatment target in CRSwNP.
Insights
Matrix metalloproteinase-9 (MMP-9) mRNA is upregulated in chronic rhinosinusitis with nasal polyps (CRSwNP), suggesting its role in disease pathogenesis. MMP-9 may be a therapeutic target for CRSwNP.
Area of Science:
- Otorhinolaryngology
- Molecular Biology
- Pathology
Background:
- Matrix metalloproteinases (MMPs) play a role in tissue remodeling.
- Chronic rhinosinusitis with nasal polyps (CRSwNP) involves significant tissue remodeling.
- MMP-9 and EMMPRIN are key regulators of extracellular matrix turnover.
Purpose of the Study:
- To compare the expression of MMP-9 and EMMPRIN in CRSwNP nasal polyps versus healthy nasal mucosa.
- To investigate the potential role of MMP-9 and EMMPRIN in CRSwNP pathogenesis.
Main Methods:
- Tissue samples were collected from 37 CRSwNP patients and 12 healthy controls.
- MMP-9 and EMMPRIN mRNA levels were quantified using RT-PCR.
- MMP-9 and EMMPRIN protein levels were assessed via Western blot analysis.
Main Results:
- MMP-9 mRNA levels were significantly higher in CRSwNP tissues compared to controls (p < 0.05).
- MMP-9 protein levels showed an increasing trend in CRSwNP but did not reach statistical significance.
- No significant differences in EMMPRIN mRNA or protein levels were observed between CRSwNP and control groups.
Conclusions:
- Elevated MMP-9 mRNA in CRSwNP indicates its involvement in the disease.
- The discrepancy between MMP-9 mRNA and protein levels suggests post-transcriptional regulation.
- While EMMPRIN showed no significant difference, its role in CRSwNP warrants further investigation.
- MMP-9 presents a potential biomarker and therapeutic target for CRSwNP.
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