Cloning, purification and characterization of human dentine matrix protein 1(DMP1)
Tarek El-Bialy1, Dror Aizenbud2, Hamid Sadeghi3
1Division Orthodontics, School of Dentistry, Faculty of Medicine and Dentistry, University of Alberta, Edmonton, AB, Canada.
The Saudi Dental Journal
|July 31, 2023
Abstract
Introduction:
Human teeth are composed mainly of dentin, formed by the odontoblasts. Dentin matrix protein 1 (DMP1) is one of odontoblast differentiation's most important growth factors. Human DMP1 has yet to be completely identified or studied. This study aimed to clone and characterize human DMP1.
Materials And Methods:
The DMP1 gene sequence was prepared and cloned by transfection of human 293 cells.
Results:
The recombinant DMP1 was purified and characterized.
Conclusion:
The results suggested its future use in dental tissue regeneration and tissue engineering.
