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Updated: Jul 20, 2025

A Customizable Approach for the Enzymatic Production and Purification of Diterpenoid Natural Products
Published on: October 4, 2019
Engineering yeast for the production of plant terpenoids using synthetic biology approaches
Jean-Alexandre Bureau1, Magdalena Escobar Oliva1, Yueming Dong1
1Department of Bioengineering, McGill University, Montreal, QC, H3A 0C3, Canada. codruta.ignea@mcgill.ca.
Abstract:
Covering: 2011-2022The low amounts of terpenoids produced in plants and the difficulty in synthesizing these complex structures have stimulated the production of terpenoid compounds in microbial hosts by metabolic engineering and synthetic biology approaches. Advances in engineering yeast for terpenoid production will be covered in this review focusing on four directions: (1) manipulation of host metabolism, (2) rewiring and reconstructing metabolic pathways, (3) engineering the catalytic activity, substrate selectivity and product specificity of biosynthetic enzymes, and (4) localizing terpenoid production via enzymatic fusions and scaffolds, or subcellular compartmentalization.
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