Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Strand exchange in site-specific recombination.

L W Enquist, H Nash, R A Weisberg

    Proceedings of the National Academy of Sciences of the United States of America
    |March 1, 1979
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Virulent Pseudorabies Virus Infection Induces a Specific and Lethal Systemic Inflammatory Response in Mice.

    Journal of virology·2018
    Same author

    The big ASM tent: how to represent the microbial sciences?

    mBio·2015
    Same author

    Us9-Independent Axonal Sorting and Transport of the Pseudorabies Virus Glycoprotein gM.

    Journal of virology·2015
    Same author

    Rapid genome assembly and comparison decode intrastrain variation in human alphaherpesviruses.

    mBio·2015
    Same author

    Genome Sequence of the Anterograde-Spread-Defective Herpes Simplex Virus 1 Strain MacIntyre.

    Genome announcements·2014
    Same author

    Making the case: married versus separate models of alphaherpes virus anterograde transport in axons.

    Reviews in medical virology·2012
    Same journal

    Tau protein as a regulator of mitochondrial function and dynamics.

    Proceedings of the National Academy of Sciences of the United States of America·2026
    Same journal

    A scalable, dividing cell model for the robust propagation and quantification of human sporadic Creutzfeldt-Jakob disease prions.

    Proceedings of the National Academy of Sciences of the United States of America·2026
    Same journal

    Epigenetic regulation of mesenchymal BMP signaling directs postnatal organ innervation.

    Proceedings of the National Academy of Sciences of the United States of America·2026
    Same journal

    Single-shot wide-field biochemical imaging at 1 kHz frame rate.

    Proceedings of the National Academy of Sciences of the United States of America·2026
    Same journal

    Morphogenesis and topological evolution of a frustrated nematic liquid crystal under confinement.

    Proceedings of the National Academy of Sciences of the United States of America·2026
    Same journal

    B cell-intrinsic CXCR3 drives efficient generation of ectopic pulmonary germinal center responses to influenza A virus infection.

    Proceedings of the National Academy of Sciences of the United States of America·2026
    See all related articles

    Site-specific recombination in phage lambda can lead to crossovers near the attachment site (att). This process requires specific phage and host genes and intact attachment sites, suggesting a novel mechanism involving strand exchange.

    Area of Science:

    • Molecular Biology
    • Genetics
    • Virology

    Background:

    • The phage lambda site-specific recombination system mediates DNA integration at the attachment site (att).
    • Understanding the precise mechanisms and boundaries of this recombination is crucial for viral genetics.

    Purpose of the Study:

    • To investigate the occurrence and requirements of crossovers outside the canonical attachment site during phage lambda site-specific recombination.
    • To elucidate the molecular mechanisms underlying these off-site recombination events.

    Main Methods:

    • Phage lambda crosses were performed under conditions selectively activating the site-specific recombination system.
    • Analysis of crossover frequencies in regions adjacent to the attachment site.
    • Genetic analysis involving phage int gene, host hip gene, and variations in attachment sites (attP vs. attB).

    Related Experiment Videos

    Main Results:

    • A low frequency of crossovers was detected in a region adjacent to, but not containing, the attachment site (att).
    • These off-site crossovers were dependent on the phage int gene, the host hip gene, and the integrity of the att site.
    • Crossovers were abolished when a heterologous attachment site (attB) replaced the native attP site.

    Conclusions:

    • Site-specific recombination at att may involve single-strand exchange and junction migration beyond the att site.
    • This suggests a broader mechanism for phage lambda integration than previously understood.
    • The findings highlight the importance of both phage and host factors in defining recombination boundaries.