Electrochemiluminescent determination of sphingomyelin in milk based on polyaniline hydrogel coupled with...

In this paper, sphingomyelin (SM) is detected by a polyaniline hydrogel and Au nanoparticles with enzyme modified electrode (GCE/PAniH/AuNPs@enzyme). After a battery of enzymic degradation, SM can generate H₂O₂ and enhance the electrochemiluminescence (ECL) response of luminol, which endows the sensor with good sensitivity, specifiity and repeatability. Additionally, the proposed ECL biosensor displays good analytical performances with a wide range from 10.0 μg·mL^-1 to 250.0 μg·mL^-1 as well as a low detection limit of 3.50 μg·mL^-1 (S/N = 3). When the ECL biosensor is used in the detection of SM in milk samples, satisfactory results are obtained, indicating that PAniH/AuNPs@enzyme will serve as a promising ECL material in the applications of H₂O₂-related bioassay in the future.