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Related Experiment Video

Updated: Jun 17, 2026

Mouse in Utero Electroporation: Controlled Spatiotemporal Gene Transfection

Mouse in Utero Electroporation: Controlled Spatiotemporal Gene Transfection

Published on: August 15, 2011

An electrogenetic interface to program mammalian gene expression by direct current.

Jinbo Huang¹, Shuai Xue¹, Peter Buchmann¹

¹Department of Biosystems Science and Engineering, ETH Zurich, Basel, Switzerland.

Nature Metabolism

|July 31, 2023

Summary

This summary is machine-generated.

Researchers developed a novel electrogenetic interface, direct current (DC)-actuated regulation technology (DART), enabling wearable devices to control gene expression. This breakthrough allows for direct programming of metabolic interventions, like insulin release, using simple DC power.

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Last Updated: Jun 17, 2026

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Published on: August 15, 2011

Culture of Embryonic Mouse Cochlear Explants and Gene Transfer by Electroporation

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Published on: January 12, 2015

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Published on: May 7, 2018

Area of Science:

Biomedical Engineering
Synthetic Biology
Wearable Technology

Background:

Wearable devices collect health data but lack direct gene therapy control.
A direct electrogenetic interface is needed to bridge this gap.
Current methods cannot directly program gene-based therapies via wearables.

Purpose of the Study:

To develop a direct electrogenetic interface for gene therapy programming.
To enable wearable devices to control transgene expression.
To demonstrate a novel method for personalized metabolic interventions.

Main Methods:

Development of direct current (DC)-actuated regulation technology (DART).
Utilizing DC power to generate reactive oxygen species for biosensor-mediated promoter regulation.
Proof-of-concept study in a type 1 diabetic mouse model using transdermal stimulation.

Main Results:

DART enables electrode-mediated, time- and voltage-dependent transgene expression.
Non-toxic reactive oxygen species reversibly fine-tune synthetic promoters.
Transdermal stimulation in diabetic mice restored normoglycemia by stimulating insulin release.

Conclusions:

DART provides the missing electrogenetic interface for wearable devices.
This technology enables direct programming of metabolic interventions.
Future applications include personalized therapies controlled by wearable electronics.