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Updated: Jul 20, 2025

Restraint to Induce Stress in Mice and Rats
Published on: December 6, 2024
Housing relocation does not have to induce a significant stress response in captive Japanese macaques (Macaca
Nelson Broche1,2, Vanessa Gris3,4, Naoko Suda-Hashimoto3,4
1Social Systems Evolution Section, Primate Research Institute, Kyoto University, Inuyama, Japan.
Background:
Previous studies suggest that housing relocation may be stressful for captive non-human primates. Our study investigated the stress levels of Japanese macaques during a housing relocation by measuring their daily fecal cortisol metabolites, which are often used as an indicator of stress.
Methods:
Ten adult Japanese macaques, single-housed for research purposes, were relocated to a new facility. Fecal samples were collected daily for 7 days. Cortisol metabolite concentrations were determined via enzyme immunoassay.
Results:
No significant differences in cortisol metabolite levels were found in 7 days, but concentration levels showed that the highest median was associated to the relocation day.
Conclusions:
The minimal cortisol metabolite increase suggests that there was a slight activity increase in the hypothalamic-pituitary-adrenal axis. Techniques encouraging cooperation of the monkeys, the short time duration of the relocation, and consistency in the environment may have contributed to the minimal stress levels observed.
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