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Related Experiment Videos

The split ejaculate: assessment of fertility potential using two in vitro test systems.

R Z Sokol, C I Madding, D J Handelsman

    Andrologia
    |July 1, 1986
    PubMed
    Summary

    Semen analysis shows sperm concentration is higher in the first ejaculate fraction. However, sperm motility and concentration correlate with cervical mucus penetration, not hamster egg penetration, in both fertile and infertile men.

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    Area of Science:

    • Reproductive biology
    • Andrology
    • Sperm function testing

    Background:

    • Semen analysis is crucial for evaluating male fertility.
    • Spermatozoa distribution within an ejaculate may impact fertility outcomes.
    • Assessing sperm function through penetration assays is vital for infertility diagnosis.

    Purpose of the Study:

    • To investigate the distribution of motile sperm concentration in split ejaculates.
    • To evaluate the correlation between sperm parameters and penetration assays (bovine cervical mucus and hamster ova).
    • To compare sperm function in fertile versus oligospermic men.

    Main Methods:

    • Semen samples from 12 normal and 8 oligospermic men were analyzed.
    • Standard semen analysis, bovine cervical mucus penetration assay (CMPT), and hamster spermatozoa zona-free penetration assay (SPA) were performed.

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  • Sperm parameters were compared between the first (FI) and second (FII) fractions of split ejaculates and whole ejaculates.

    • Main Results:

    • In normal men, motile sperm concentration was significantly higher in FI than FII.
    • In oligospermic men, sperm and motile sperm concentrations were higher in FI than FII.
    • Motile sperm concentration correlated significantly with CMPT in both groups, but not with SPA.

    Conclusions:

    • The first fraction of ejaculate contains a higher concentration of motile sperm.
    • Sperm motility and concentration are predictive of cervical mucus penetration ability.
    • Zona-free hamster egg penetration assay may not be a reliable indicator of fertility in these patient groups.