Difunctional Fluorescent Probes for Iron and Hydrogen Sulfide Detection Based on Diphenyl Derivative

In order to better monitor the content of Fe³⁺ and H₂S in the biological environment, two new fluorescent probes were designed and synthesized. With the addition of Fe³⁺, the strong fluorescence emission of two probes was significantly quenched due to the paramagnetic effect of Fe³⁺. With the further addition of S^2-, the fluorescence intensity was quickly restored. Two probes showed high selectivity and strong sensitivity for the detection of Fe³⁺ and S^2-, and the fluorescence intensity "ON-OFF-ON" was accompanied with the interaction process. At the same time, two probes displayed good anti-interference ability which was not interfered by the existence of other ions. In addition, two probes illustrated fast response time to Fe³⁺, S^2- and small cytotoxicity to cells. Therefore, two probes can provide a potential ideal tool for detecting Fe³⁺ and H₂S in organisms and the environment.