Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Next-generation Sequencing03:00

Next-generation Sequencing

91.5K
The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features....
91.5K
Sanger Sequencing01:57

Sanger Sequencing

754.8K
DNA sequencing is a fundamental technique that is routinely used in the biological sciences. This method can be applied to a range of questions at different scales - from the sequencing of a cloned DNA fragment or the study of a mutation in a gene up to whole-genome sequencing. However, despite the widespread use of sequencing today, it was not until 1977 that Fredrick Sanger and his collaborators developed the chain-termination method to decode DNA sequences. It relies on the separation of a...
754.8K
Maxam-Gilbert Sequencing01:05

Maxam-Gilbert Sequencing

11.2K
In the same year as the discovery of the Sanger sequencing method, another group of scientists, Allan Maxam and Walter Gilbert, demonstrated their chemical-cleavage method for DNA sequencing. The Maxam-Gilbert method relies on using different chemicals that can cleave the DNA sequence at specific sites, the separation of resulting DNA fragments of variable size using electrophoresis, and deciphering the DNA sequence from the resulting gel bands.
Challenges of the Maxam-Gilbert Method
The...
11.2K
Genomics02:02

Genomics

36.5K
Genomics is the science of genomes: it is the study of all the genetic material of an organism. In humans, the genome consists of information carried in 23 pairs of chromosomes in the nucleus, as well as mitochondrial DNA. In genomics, both coding and non-coding DNA is sequenced and analyzed. Genomics allows a better understanding of all living things, their evolution, and their diversity. It has a myriad of uses: for example, to build phylogenetic trees, to improve productivity and...
36.5K
Modern Molecular Taxonomy01:29

Modern Molecular Taxonomy

52
Advancements in molecular biology have revolutionized the identification and characterization of bacteria, with multiple methods leveraging DNA sequencing for enhanced precision. As sequencing technologies improve and costs decline, these approaches are increasingly used in clinical, environmental, and evolutionary studies.Multilocus Sequence Typing (MLST) examines several housekeeping genes, essential chromosomal genes encoding cellular functions, to distinguish strains. Approximately...
52
RNA-seq03:21

RNA-seq

10.1K
RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
10.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Clinical Predictors of Fatal Outcomes from Human Leptospirosis, Thailand, 2015-2024.

Emerging infectious diseases·2026
Same author

Clinical and molecular characterization of an outbreak of leptospirosis in dogs from Los Angeles County, California, USA, 2021.

Journal of clinical microbiology·2026
Same author

Cellulosimicrobium Sepsis: Identification of an Emerging Pathogen Using Whole-Genome Sequencing.

The American journal of tropical medicine and hygiene·2026
Same author

Bloodmeal analysis via COI-targeted DNA capture and enrichment identifies non-cattle hosts of Amblyomma variegatum on St. Croix, US Virgin Islands.

Parasites & vectors·2026
Same author

Emergence of Human and Animal Melioidosis in Southern Africa, 2018-2021.

Tropical medicine and infectious disease·2026
Same author

Linking the epidemiology of coccidioidomycosis and environmental exposure through targeted genomic enrichment of <i>Coccidioides posadasii</i>.

mBio·2025

Related Experiment Video

Updated: Jul 20, 2025

Author Spotlight: A Cost-Effective Genomic Workflow for Advancing Rabies Control in Resource-Limited Settings
10:26

Author Spotlight: A Cost-Effective Genomic Workflow for Advancing Rabies Control in Resource-Limited Settings

Published on: August 18, 2023

5.3K

Towards a post-pandemic future for global pathogen genome sequencing.

Jason T Ladner1,2, Jason W Sahl1,2

  • 1The Pathogen and Microbiome Institute, Northern Arizona University, Flagstaff, Arizona, United States of America.

Plos Biology
|August 1, 2023
PubMed
Summary
This summary is machine-generated.

Routine pathogen genome sequencing aids infectious disease outbreak response and epidemic preparedness. Advances enable tracking disease spread, with the SARS-CoV-2 pandemic highlighting its crucial role and future challenges.

More Related Videos

Next-generation Sequencing of 16S Ribosomal RNA Gene Amplicons
10:24

Next-generation Sequencing of 16S Ribosomal RNA Gene Amplicons

Published on: August 29, 2014

83.5K
Validating Whole Genome Nanopore Sequencing, using Usutu Virus as an Example
05:45

Validating Whole Genome Nanopore Sequencing, using Usutu Virus as an Example

Published on: March 11, 2020

8.8K

Related Experiment Videos

Last Updated: Jul 20, 2025

Author Spotlight: A Cost-Effective Genomic Workflow for Advancing Rabies Control in Resource-Limited Settings
10:26

Author Spotlight: A Cost-Effective Genomic Workflow for Advancing Rabies Control in Resource-Limited Settings

Published on: August 18, 2023

5.3K
Next-generation Sequencing of 16S Ribosomal RNA Gene Amplicons
10:24

Next-generation Sequencing of 16S Ribosomal RNA Gene Amplicons

Published on: August 29, 2014

83.5K
Validating Whole Genome Nanopore Sequencing, using Usutu Virus as an Example
05:45

Validating Whole Genome Nanopore Sequencing, using Usutu Virus as an Example

Published on: March 11, 2020

8.8K

Area of Science:

  • Microbiology
  • Genomics
  • Epidemiology

Background:

  • Pathogen genome sequencing is vital for infectious disease outbreak management.
  • It plays a key role in preparing for future epidemics.
  • Technological advancements have made routine sequencing feasible.

Purpose of the Study:

  • To discuss innovations enabling routine pathogen genome sequencing.
  • To explain how genome sequences aid in understanding and controlling disease spread.
  • To explore the impact of the SARS-CoV-2 pandemic on pathogen genomics and identify future challenges.

Main Methods:

  • Review of innovations in pathogen genome sequencing technology.
  • Analysis of applications of pathogen genome sequences in disease control.
  • Examination of the influence of the SARS-CoV-2 pandemic on the field.

Main Results:

  • Routine pathogen genome sequencing is now a standard tool for outbreak response.
  • Genome sequences provide critical insights into disease transmission dynamics.
  • The SARS-CoV-2 pandemic accelerated advancements and highlighted the importance of pathogen genomics.

Conclusions:

  • Pathogen genome sequencing is essential for both current outbreak management and future epidemic preparedness.
  • Continued innovation and addressing existing challenges will further enhance the utility of pathogen genomics.
  • Genomic data is indispensable for effective public health interventions against infectious diseases.