Related Experiment Video

Updated: Jul 20, 2025

Measurements of Physiological Stress Responses in C. Elegans
10:36

Measurements of Physiological Stress Responses in C. Elegans

Published on: May 21, 2020

14.0K

[Inactivation of Ras1 in Fission Yeast Aggravates the Oxidative Stress Response Induced by Tert Butyl Hydroperoxide

N Masood1, S Anjum2, S Ahmed2,3

  • 1Biochemistry and Structural Biology Division, CSIR-Central Drug Research Institute, Sector 10, Jankipuram Extension, Lucknow, 226031 India.

Molekuliarnaia Biologiia
|August 2, 2023
PubMed

Insights

Schizosaccharomyces pombe Ras1 protein helps cells manage oxidative stress. Ras1 mitigates reactive oxygen species (ROS) and maintains mitochondrial function, protecting against cellular damage.

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Biochemistry

Context:

  • Ras proteins are GTPases regulating cellular homeostasis.
  • Dysregulated Ras signaling is linked to pathologies.
  • Ras proteins influence redox signaling and reactive oxygen species (ROS) levels.

Purpose:

  • To investigate the role of Ras1 in oxidative stress response in Schizosaccharomyces pombe.
  • To evaluate the effects of tert-Butyl hydroperoxide (tBHP) and antimycin A on Ras1 function.

Summary:

  • Ras1 deletion (ras1Δ) and treatment with tBHP or antimycin A decreased cell survival and increased ROS levels and mitochondrial dysfunction.
  • These defects were exacerbated in ras1Δ cells under oxidative stress.
  • Ras1 regulates antioxidant enzymes including glutathione peroxidase (GSH-Px), glutathione-S-transferase (GST), and catalase.

Impact:

  • S. pombe Ras1 plays a significant role in mitigating oxidative stress.
  • Findings suggest Ras1's involvement in cellular defense mechanisms against ROS-induced damage.
  • This study provides insights into the molecular mechanisms underlying oxidative stress response in yeast.
Keywords:
ROSRas1Schizosaccharomyces pombeoxidative stresstBHP

More Related Videos

Microarray Analysis for Saccharomyces cerevisiae13:17

Microarray Analysis for Saccharomyces cerevisiae

Published on: April 7, 2011

13.7K
Studying Oxidative Stress Caused by the Mitis Group Streptococci in Caenorhabditis elegans09:36

Studying Oxidative Stress Caused by the Mitis Group Streptococci in Caenorhabditis elegans

Published on: March 23, 2019

7.2K

Related Concept Videos

Oxygen Requirements and Growth Patterns01:29

Oxygen Requirements and Growth Patterns

Microorganisms exhibit diverse oxygen requirements and growth patterns driven by their metabolic strategies and environmental adaptations. Oxygen, while essential for many organisms, can also be toxic under certain conditions, shaping how microorganisms grow and survive.Oxygen Requirements of MicroorganismsMicroorganisms are classified based on their ability to use or tolerate oxygen:Obligate aerobes like Mycobacterium tuberculosis need oxygen for energy production, as it serves as the terminal...
161
The Ras Gene02:38

The Ras Gene

The Ras-gene-encoded proteins are regulators of signaling pathways controlling cell proliferation, differentiation, or cell survival. The Ras-gene family in humans constitutes three primary members—the HRas, NRas, and KRas. These genes code for four functionally distinct yet closely related proteins—the HRas, NRas, KRas4A, and KRas4B. The involvement of mutant Ras genes in human cancer was first discovered in 1982 and is among the most common causes of human tumorigenesis.
Ras is a...
6.3K
Peroxisomes01:24

Peroxisomes

Peroxisomes are specialized organelles present in fungi, plant, and animal cells. It can vary in number, size, morphology, and activity depending on the type of tissue and the nutritional state of the cell. For example, cells with active lipid metabolism, such as adipocytes, neurons, and hepatocytes, have more peroxisomes than other cells in the body. Besides their primary role in breaking down complex organic molecules, peroxisomes can also synthesize specific macromolecules and participate in...
13.6K
Radical Autoxidation01:20

Radical Autoxidation

The oxidation of an organic compound in the presence of air or oxygen is called autoxidation. For example, cumene reacts with oxygen to form hydroperoxide. Autoxidation involves initiation, propagation, and termination steps. Many organic compounds are susceptible to autoxidation—especially ethers in the presence of oxygen, which form hydroperoxides. Even though this reaction is slow, old ether bottles contain small amounts of peroxide, which leads to laboratory explosions during ether...
2.2K
Other Stress Responses in Bacteria01:30

Other Stress Responses in Bacteria

Bacteria have global regulatory systems that control several types of stress mechanisms. These include Pho regulon and the heat shock response, which are essential systems for environmental adaptation, such as nutrient limitation and proteotoxic stress. The Pho regulon and the heat shock response exemplify bacterial resilience, enabling rapid adaptation to fluctuating environmental conditions.Pho RegulonBacteria require phosphorus for essential cellular processes, including nucleic acid...
33