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Updated: Jul 20, 2025
07:54
Manipulation of Ploidy in Caenorhabditis elegans
Published on: March 15, 2018
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Engineering homoeologs provide a fine scale for quantitative traits in polyploid.
Eun Song Lee1, Jung Heo1,2, Woo Young Bang3
1Division of Biological Sciences, Wonkwang University, Iksan, Korea.
Plant Biotechnology Journal
|August 2, 2023
Summary
Genome editing in polyploid crops like black nightshade (Solanum nigrum) is now possible. Engineering homoeologous genes in this hexaploid species improved fruit productivity, offering agricultural benefits.
Area of Science:
- Genomics
- Plant Breeding
- Molecular Biology
Background:
- Polyploidy is common in staple crops, posing challenges for genetic modification.
- Recent advancements in genome sequencing and editing technologies facilitate polyploid genome engineering.
Purpose of the Study:
- To assemble the genome of the hexaploid black nightshade (Solanum nigrum).
- To identify and engineer homoeologous genes for agricultural improvement.
Main Methods:
- Scaffold-level genome assembly and functional annotation of Solanum nigrum.
- Comparative analysis of orthologous genes with diploid relatives (Solanum americanum, Solanum lycopersicum).
- CRISPR-Cas9-mediated mutagenesis of homoeologous genes.
Main Results:
- Identification of homoeologous gene sets with similar sequence and expression profiles.
- Generation of multiple mutants with quantitative phenotypic changes in hexaploid S. nigrum.
- Successful improvement of fruit productivity in the Boranong cultivar of S. nigrum.
Conclusions:
- Engineering homoeologous genes offers a viable strategy for the agricultural improvement of polyploid crops.
- This approach can enhance quantitative traits and productivity in species like black nightshade.
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