Detection of Doxycycline Using Carbon Quantum dots as Probe Based on Internal Filtering Effect

Area of Science:

• Analytical Chemistry
• Materials Science
• Biomedical Engineering

Background:

• Doxycycline (DC) monitoring is crucial for drug safety and residue assessment.
• Existing detection methods may lack convenience or effectiveness.
• Novel fluorescent probes are needed for sensitive DC detection.

Purpose of the Study:

• To develop a simple, rapid, and effective fluorescence-based detection method for doxycycline.
• To utilize carbon quantum dots (CQDs) as sensitive fluorescent probes for DC.
• To establish a quantitative analytical method for DC determination in pharmaceutical and biological samples.

Main Methods:

• Synthesis of carbon quantum dots (CQDs) via a melting method.
• Development of a fluorescence analytical method based on the internal filtration effect (IFE).
• Verification of the quenching mechanism using fluorescence lifetime and absorption spectroscopy.

Main Results:

• CQDs exhibited stable luminescence with a quantum yield of 21.8%.
• A linear relationship was observed between DC concentration (5-30 µM) and fluorescence quenching efficiency (R²=0.9987).
• The method achieved a detection limit of 2.343 µM and was successfully applied to tablets and serum.

Conclusions:

• The developed fluorescence method provides a promising approach for doxycycline detection.
• The method's simplicity, rapidity, and accuracy make it suitable for biomedical applications.
• This technique aids in drug monitoring and quality control of doxycycline.