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Updated: Jul 20, 2025
DNA Virus Detection System Based on RPA-CRISPR/Cas12a-SPM and Deep Learning
Published on: May 10, 2024
Extraction-free, one-pot CRISPR/Cas12a detection of microRNAs directly from extracellular vesicles
He Yan1, Song Han2, Steven Hughes2
1Department of Chemistry, University of Florida, Gainesville, FL 32611, USA. zengy@ufl.edu.
None:
Current methods for extracellular vesicle (EV) miRNA analysis mostly require RNA extraction, which results in a multi-step, time-consuming workflow. This study reports an extraction-free method that combines thermolysis treatment of EVs with a one-pot EXTRA-CRISPR assay, enabling the vastly simplified analysis of EV miRNAs with a comparable performance to that of the extraction-based assays.
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