Improved endotoxin removal using ecofriendly detergents for intensified plasmid capture

Area of Science:

• Biotechnology
• Process Chemistry

Background:

• Growing demand for plasmid DNA in gene therapies and vaccines highlights production bottlenecks.
• Traditional bead-based chromatography is a key limitation in plasmid DNA purification.
• Need for efficient, scalable methods with effective impurity clearance.

Purpose of the Study:

• To establish an intensified plasmid DNA capture process using convective anion exchange (AEX) chromatography.
• To evaluate sustainable detergent alternatives for enhanced endotoxin clearance.
• To assess process compatibility and impurity removal for diverse plasmid types.

Main Methods:

• Utilized convective AEX chromatography with membrane and monolith supports.
• Screened various detergents across different physico-chemical classes.
• Assessed purification efficiency, including endotoxin and host cell protein (HCP) clearance, plasmid yield, and analytical interference.

Main Results:

• Demonstrated efficient plasmid DNA capture using convective AEX chromatography.
• Identified environmentally benign detergents that enhance endotoxin clearance.
• Evaluated the impact of detergents on plasmid yield and process compatibility.

Conclusions:

• Convective AEX chromatography offers an intensified solution for plasmid DNA production.
• Sustainable detergents can improve impurity removal without compromising yield or process integration.
• This approach provides insights for optimizing large-scale plasmid DNA manufacturing.