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Transcriptomic profiling of mice brain under Bex3 regulation.

Zhimin Xu1, Noor Bahadar2,3, Yingxin Zhang4

  • 1Department of Oral and Maxillofacial Surgery, School and Hospital of Stomatology, Jilin University, Changchun, China.

Turkish Journal of Biology = Turk Biyoloji Dergisi
|August 3, 2023
PubMed
Summary

The Bex3 gene is crucial for brain development. Knocking out Bex3 in mice altered gene expression, impacting neuronal functions and identifying links to intellectual disability.

Keywords:
CRISPR-Cas9KEGG pathwaygene ontology analysistranscriptome analysistransgenic mouse model

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Area of Science:

  • Neuroscience
  • Genetics
  • Molecular Biology

Background:

  • BEX family genes are vital for neuronal development.
  • The specific role of Bex3 in the brain requires further elucidation.

Purpose of the Study:

  • To investigate the function of Bex3 in brain development using a knockout mouse model.
  • To identify genes and pathways regulated by Bex3.

Main Methods:

  • CRISPR-Cas9 gene editing was used to create a Bex3 knockout mouse model.
  • Transcriptomic analysis of the brain was performed to assess gene expression changes.

Main Results:

  • Bex3 knockout altered the expression of 95 genes (53 down, 42 up), including those related to neuronal function, hippocampus, and cortex.
  • Enrichment analysis revealed significant associations with non-syndromic X-linked mental retardation and various transcription factor families (IRF, MBD, SAND, zf-BED, zf-C2H2).

Conclusions:

  • Bex3 plays a significant role in regulating gene expression critical for brain development.
  • The study identifies potential molecular pathways linking Bex3 dysfunction to intellectual disability, warranting further investigation.