Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Gene-dependent flavonoid 3'-hydroxylation in maize.

R Larson, J B Bussard, E H Coe

    Biochemical Genetics
    |August 1, 1986
    PubMed
    Summary

    The gene Pr in maize directly influences flavonoid 3'-hydroxylase activity, controlling pigment production. Higher Pr gene dosage increases hydroxylase activity and the cyanidin to pelargonidin ratio, suggesting Pr is a structural gene.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Radioactive (Pu, <sup>210</sup>Pb, <sup>234</sup>Th and <sup>226</sup>Ra) and chemical (Mn, Fe, Al) tracers to evaluate sediment accumulation and biodiffusion in the southern Gulf of Mexico.

    Journal of environmental radioactivity·2026
    Same author

    A systematic review and network meta-analysis of injectable antibiotic options for the control of bovine respiratory disease in the first 45 days post arrival at the feedlot.

    Animal health research reviews·2020
    Same author

    A systematic review and network meta-analysis of bacterial and viral vaccines, administered at or near arrival at the feedlot, for control of bovine respiratory disease in beef cattle.

    Animal health research reviews·2020
    Same author

    Corrigendum to "Grazing intensity affects the environmental impact of dairy systems" (J. Dairy Sci. 100:6804-6821).

    Journal of dairy science·2018
    Same author

    Grazing intensity affects the environmental impact of dairy systems.

    Journal of dairy science·2017
    Same author

    What is the Most Cost-Effective Strategy for Treating Chronic Myeloid Leukemia After Imatinib Loses Patent Exclusivity in Europe?

    Value in health : the journal of the International Society for Pharmacoeconomics and Outcomes Research·2016

    Area of Science:

    • Plant genetics
    • Biochemistry
    • Maize gene expression

    Background:

    • Flavonoid biosynthesis pathways are crucial for plant pigmentation.
    • The Pr gene's role in regulating flavonoid 3 ahydroxylase activity in maize was previously unclear.

    Purpose of the Study:

    • To investigate the influence of the Pr gene dosage on flavonoid 3 ahydroxylase activity in maize.
    • To determine if Pr is the structural gene for this hydroxylase.

    Main Methods:

    • Enzyme activity assays in maize seedlings and aleurone tissue with varying Pr genotypes.
    • Quantitative analysis of cyanidin and pelargonidin pigments.
    • Regression analysis to correlate gene dosage with enzyme activity and pigment ratios.

    Main Results:

    • Flavonoid 3 ahydroxylase activity increased proportionally with Pr gene dosage in both seedlings and aleurone.
    • The ratio of cyanidin to pelargonidin was strongly correlated with Pr gene dosage.
    • Cyanidin levels increased while pelargonidin decreased with higher Pr dosage.

    Conclusions:

    • The Pr gene functions as the structural gene for NADPH:flavonoid 3 ahydroxylase in maize.
    • Pr gene dosage precisely controls the ratio of cyanidin to pelargonidin, impacting flavonoid profiles.
    • Low activity in pr homozygous tissue suggests potential hypomorphic alleles or duplicate genes.

    Related Experiment Videos