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Enhanced Maturation of 3D Bioprinted Skeletal Muscle Tissue Constructs Encapsulating Soluble Factor-Releasing
Natan Roberto de Barros1, Mohammad Ali Darabi1, Xin Ma2
1Terasaki Institute for Biomedical Innovation (TIBI), Los Angeles, CA, 90064, USA.
Macromolecular Bioscience
|August 3, 2023
Summary
This study developed a 3D bioprinting method using a novel bioink for skeletal muscle tissue engineering. The engineered muscle tissues showed enhanced myotube maturation and spontaneous contraction, paving the way for advanced muscle regeneration therapies.
Area of Science:
- Biomaterials Science
- Tissue Engineering
- Regenerative Medicine
Background:
- Skeletal muscle tissue engineering faces challenges in creating native-like, functional muscle constructs.
- Existing methods struggle with cell alignment, differentiation, and maturation for effective muscle regeneration.
Purpose of the Study:
- To develop an advanced 3D bioprinting strategy for skeletal muscle tissue engineering.
- To investigate the efficacy of a novel bioink incorporating sustained insulin-like growth factor-1 (IGF-1) release for enhanced muscle development.
Main Methods:
- Fabrication of muscle tissue constructs using 3D bioprinting with gelatin methacryloyl (GelMA) bioink.
- Incorporation of microfluidic-assisted polymeric microparticles for sustained release of insulin-like growth factor-1 (IGF-1).
- Evaluation of myoblast alignment, differentiation, myotube maturation, and functional contraction in engineered muscle tissues.
Main Results:
- Synthesized microparticles demonstrated successful IGF-1 adsorption and sustained release for over 21 days.
- The IGF-1-releasing GelMA bioink promoted myoblast alignment and differentiation into mature myotubes.
- Engineered muscle constructs exhibited spontaneous myotube contraction, indicating functional tissue development.
Conclusions:
- The proposed 3D bioprinting strategy using IGF-1-releasing microparticles significantly enhances in vitro skeletal muscle engineering.
- This approach holds promise for developing improved therapeutic strategies for muscle tissue regeneration and maturation.
