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System analysis identifies UBE2C as a novel oncogene target for adrenocortical carcinoma

Renlun Huang1,2, Lang Guo1,3, Chiwei Chen1,2

  • 1The Research Center of Integrative Cancer Medicine, Discipline of Integrated Chinese and Western Medicine, The Second Clinical College of Guangzhou University of Chinese Medicine, Guangzhou, Guangdong, China.

Plos One
|August 3, 2023
PubMed

Insights

Ubiquitin Conjugating Enzyme 2C (UBE2C) drives tumor progression in adrenocortical carcinoma (ACC). High UBE2C expression correlates with poor prognosis, promoting cell cycle and EMT, making it a potential therapeutic target.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Ubiquitin Conjugating Enzyme 2C (UBE2C) is implicated in tumor progression.
  • The specific role and mechanism of UBE2C in adrenocortical carcinoma (ACC) are not well understood.
  • Investigating UBE2C's tumorigenic effects could clarify its prognostic value in ACC.

Purpose of the Study:

  • To systematically investigate the tumorigenic effect and prognostic value of UBE2C in adrenocortical carcinoma (ACC).
  • To explore the underlying mechanisms by which UBE2C influences ACC progression, including cell cycle and epithelial-mesenchymal transition (EMT).

Main Methods:

  • Utilized bioinformatics analysis of The Cancer Genome Atlas (TCGA) RNA-seq data for ACC via ACLBI Web-based Tools.
  • Identified candidate genes by intersecting DFS-related genes, OS-related genes, highly expressed genes in ACC, and differentially expressed genes (DEGs).
  • Performed in vitro experiments involving UBE2C knockdown in ACC cells to assess proliferation, migration, invasion, EMT, and cell cycle progression.

Main Results:

  • UBE2C was identified as the most significant DEG between ACC tumor and normal tissues, with high expression linked to poor prognosis.
  • UBE2C expression positively correlated with cell cycle regulators (CDC20, CDK1, CCNA2), indicating hyperactive cell cycle progression.
  • UBE2C knockdown significantly inhibited ACC cell proliferation, migration, invasion, EMT, and cell cycle progression in vitro.
  • Pan-cancer analysis revealed UBE2C as an oncogene across multiple tumor types.

Conclusions:

  • High UBE2C expression is strongly associated with poor prognosis in ACC patients.
  • UBE2C promotes ACC progression by enhancing cell cycle progression and EMT.
  • UBE2C represents a promising molecular target for novel ACC therapies.

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