Related Experiment Video
Updated: Jul 20, 2025

Efficient Transfection of In vitro Transcribed mRNA in Cultured Cells Using Peptide-Poloxamine Nanoparticles
Published on: August 17, 2022
The mRNA Vaccine Revolution: COVID-19 Has Launched the Future of Vaccinology
Zhongfeng Ye1, Joseph Harmon1, Wei Ni2
1Department of Biomedical Engineering, Tufts University, Medford, Massachusetts 02155, United States.
Abstract:
During the COVID-19 pandemic, mRNA (mRNA) vaccines emerged as leading vaccine candidates in a record time. Nonreplicating mRNA (NRM) and self-amplifying mRNA (SAM) technologies have been developed into high-performing and clinically viable vaccines against a range of infectious agents, notably SARS-CoV-2. mRNA vaccines demonstrate efficient in vivo delivery, long-lasting stability, and nonexistent risk of infection. The stability and translational efficiency of in vitro transcription (IVT)-mRNA can be further increased by modulating its structural elements. In this review, we present a comprehensive overview of the recent advances, key applications, and future challenges in the field of mRNA-based vaccinology.
Related Concept Videos
Microorganisms in Medicine and Therapeutics
Vaccinations
The Central Dogma
RNA is the Missing Link Between DNA and Proteins
In the early 1900s, scientists discovered that DNA stores all the information needed for cellular functions and that proteins perform most of these functions. However, the mechanisms of converting genetic information into functional proteins remained unknown for many years. Initially, it was believed that a single gene is...
Leaky Scanning
Nuclear Export of mRNA
Viral Mutations

