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Related Experiment Videos

Assembly of overlapping DNA sequences by a program written in BASIC for 64K CP/M and MS-DOS IBM-compatible

R E Johnston, J M Mackenzie, W G Dougherty

    Nucleic Acids Research
    |January 10, 1986
    PubMed
    Summary

    This study introduces SEQALIGN, a software tool that simplifies DNA sequence assembly. It efficiently aligns up to 100 overlapping DNA sequences, aiding genetic research and analysis.

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    Area of Science:

    • Bioinformatics
    • Molecular Biology
    • Computational Genetics

    Background:

    • DNA sequencing generates large datasets requiring assembly.
    • Existing methods for DNA sequence assembly can be complex and resource-intensive.

    Purpose of the Study:

    • To present the SEQALIGN programs for DNA sequence assembly.
    • To facilitate the alignment of multiple overlapping DNA sequences.

    Main Methods:

    • Development of the SEQALIGN software using Microsoft BASIC interpreter.
    • Utilizing WORDSTAR or similar word processors for sequence data input and editing.
    • Ensuring minimal hardware requirements for execution on CP/M and MS-DOS systems.

    Main Results:

    • The SEQALIGN program successfully assembles up to 100 individual overlapping DNA sequences.

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  • It generates a printout of aligned sequences presented in register.
  • The software is user-friendly due to its BASIC interpreter and compatibility with common word processors.

    • Conclusions:

    • SEQALIGN provides an accessible and efficient solution for DNA sequence assembly.
    • The program's design lowers the barrier to entry for researchers with limited computational resources.
    • It supports standard DNA sequencing workflows, including M-13 subcloning methods.