Related Experiment Video
Updated: Aug 17, 2026
Generation of Native Chromatin Immunoprecipitation Sequencing Libraries for Nucleosome Density Analysis
Published on: December 12, 2017
Use of Native-PAGE for the Identification of Epichaperomes in Cell Lines
Tanaya Roychowdhury1, Anand R Santhaseela1, Sahil Sharma1
1Program in Chemical Biology, Memorial Sloan Kettering Cancer Center, New York, NY, USA.
Abstract:
Epichaperomes are disease-associated pathologic scaffolds, composed of tightly bound chaperones, co-chaperones, and other factors. They mediate anomalous protein-protein interactions inside cells, which aberrantly affects the function of protein networks, and in turn, cellular phenotypes. Epichaperome study necessitates the implementation of methods that retain these protein complexes in their native cellular states for analysis. Here we describe a protocol for detection and composition analysis of epichaperomes in cell homogenates through native polyacrylamide gel electrophoresis.
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