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Updated: Jul 20, 2025

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Molecular Chaperone Receptors: An Update

Thiago J Borges1, Ayesha Murshid2, Jimmy Theriault2

  • 1Center for Transplantation Sciences, Department of Surgery, Massachusetts General Hospital, Harvard Medical School, Boston, MA, USA.

Methods in Molecular Biology (Clifton, N.J.)
|August 4, 2023
PubMed

Insights

Extracellular heat shock proteins (HSP) bind to immune cell receptors. This study identifies c-type lectin receptors, scavenger receptors, and lectins as key binding partners for Hsp70, advancing our understanding of HSP-mediated signaling.

Area of Science:

  • Immunology
  • Cell Biology
  • Biochemistry

Background:

  • Extracellular heat shock proteins (HSP) are crucial for cell signaling and immune responses.
  • Surface receptors on immune cells mediate many HSP effects.
  • Understanding these receptor-ligand interactions is key to deciphering HSP functions.

Purpose of the Study:

  • To identify and characterize receptors that bind extracellular heat shock proteins (HSP).
  • To investigate the structural basis of Hsp70 interactions with its receptors.
  • To outline methods for discovering and studying HSP receptors and their functions.

Main Methods:

  • Cloning candidate receptors into HSP-binding null cells (CHO-K1).
  • Investigating binding of mammalian and eukaryotic Hsp70 to various receptor classes.
  • Analyzing receptor domains involved in Hsp70 binding, including CTLD and EGF-like repeats.

Main Results:

  • Hsp70 avidly binds to at least three receptor classes: c-type lectin receptors (CLR), scavenger receptors (SR), and lectins.
  • Hsp70 interacts with LOX-1 via its c-type lectin binding domain (CTLD).
  • Hsp70 also binds to SR family members SREC-I and FEEL-1/CLEVER-1/STABILIN-1, which possess EGF-like repeats.

Conclusions:

  • Identified CLR, SR, and lectins as major classes of Hsp70 receptors.
  • Provided insights into specific receptor-ligand interactions, such as Hsp70 with LOX-1.
  • Established a framework for discovering and studying HSP receptors and their in vivo functions.
Keywords:
ExtracellularHeatImmune suppressiveImmunityProteinReceptorScavengerShock

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