A nonenzymic microfiber optic-biosensor modified phenylboric acid for sensitively and specifically detecting low...

Area of Science:

• * Optical sensing
• * Nanomaterials
• * Biomedical diagnostics

Background:

• * Glucose detection is crucial for managing diabetes.
• * Existing methods often lack sensitivity or require complex sample preparation.
• * Development of rapid, sensitive, and cost-effective glucose sensors is needed.

Purpose of the Study:

• * To design and demonstrate a microfiber interferometer for trace glucose detection.
• * To utilize a novel coating of polydopamine (PDA) and 3-Carboxy-4-fluorophenylboronic acid (FPBA) for enhanced sensitivity.
• * To evaluate the sensor's performance in detecting glucose in human urine samples.

Main Methods:

• * Fabrication of a microfiber interferometer coated with a PDA/FPBA film.
• * Measurement of refractive index (RI) sensitivity and temperature response.
• * Glucose detection using wavelength shift analysis.
• * Validation of the sensor in human urine samples.

Main Results:

• * The microfiber interferometer exhibited high RI sensitivity (1825.83 nm/RIU) and low temperature response (-0.04 nm/°C).
• * The PDA/FPBA coating enabled glucose detection in the range of 0.1-20 mM with a sensitivity of 1.71 nm/mM.
• * A limit of detection of 12.6 ppm was achieved.
• * The sensor demonstrated responsiveness and reproducibility in detecting glucose in human urine.

Conclusions:

• * The developed sensor offers a simple, low-cost, and highly sensitive method for glucose detection.
• * The combination of microfiber technology and organic chemical materials shows promise for optical detection applications.
• * This sensor is suitable for estimating glucose concentration in urine, aiding in diabetes management.