Sustainable mixotrophic microalgae refinery of astaxanthin and lipid from Chlorella zofingiensis

Microalgal astaxanthin possesses numerous bioactivities and has several health applications. The current research focuses on designing and optimizing the two-stage mixotrophic bioprocess by Chlorella zofingiensis for astaxanthin production. Gradual increase in light intensity (4-8k-lux) and 3x micronutrient concentration were the key parameters for maximizing biomass yield of 2.5 g/L during 15 days of stage I. Furthermore, stress conditions (excessive CO_2, light, salinity, etc.) enhanced astaxanthin yield at stage II. 20k lux light, 3x nutrients, and 5% CO₂ were the best ranges for maximum astaxanthin production. Maximum biomass yield and astaxanthin content were 3.3 g/L and 16.7 mg/g, respectively, after 29 days of bioprocess. Astaxanthin biosynthesis was also affected by salinity, but less than other parameters. Astaxanthin bioprocess resulted in enhanced lipid yields of 35-37%, which could be used for biodiesel. This study shows promising scale-up potential with attractive sustainability features of Chlorella zofingiensis model for commercial astaxanthin-lipid biorefinery.