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Related Concept Videos

2° Amines to N-Nitrosamines: Reaction with NaNO201:20

2° Amines to N-Nitrosamines: Reaction with NaNO2

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Secondary amines react with nitrous acid to form N-nitrosamines, as depicted in Figure 1. Nitrous acid, a weak and unstable acid, is formed in situ from an aqueous solution of sodium nitrite and strong acids, such as hydrochloric acid or sulfuric acid, in cold conditions. In the presence of an acid, the nitrous acid gets protonated. The subsequent loss of water results in the formation of the electrophile known as nitrosonium ion.
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Inorganic Nitrogen Assimilation01:22

Inorganic Nitrogen Assimilation

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Nitrogen is an essential element in biological systems, forming a crucial component of proteins, nucleic acids, and other cellular constituents. Many bacteria and archaea acquire nitrogen in the form of nitrate (NO₃⁻) or ammonia (NH₃), which are then assimilated into biomolecules through specific enzymatic pathways.Assimilatory Nitrate ReductionWhen nitrate enters the cell, it undergoes a two-step reduction process known as assimilatory nitrate reduction. Initially, the enzyme...
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1° Amines to Diazonium or Aryldiazonium Salts: Diazotization with NaNO2 Mechanism01:37

1° Amines to Diazonium or Aryldiazonium Salts: Diazotization with NaNO2 Mechanism

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Nitrous acid is a relatively weak and unstable acid prepared in situ by the reaction of sodium nitrite and cold, dilute hydrochloric acid. In an acidic solution, the nitrous acid undergoes protonation when it loses water to form a nitrosonium ion—an electrophile. Nitrous acid reacts with primary amines to give diazonium salts. The reaction is called diazotization of primary amines.
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1° Amines to Diazonium or Aryldiazonium Salts: Diazotization with NaNO2 Overview01:26

1° Amines to Diazonium or Aryldiazonium Salts: Diazotization with NaNO2 Overview

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Nitrous acid and nitric acids are two types of acids containing nitrogen, among which nitrous acid is weaker than nitric acid. Nitrous acid with a pKa value of 3.37 ionizes in water to give a nitrite ion and the hydronium ion.
The nitrous acid is unstable. Hence, it is formed in situ from a solution of sodium nitrite and cold aqueous acids such as hydrochloric or sulfuric acid. In an acidic solution, the –OH group of nitrous acid undergoes protonation to give oxonium ion, followed by...
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Overview of Nitrogen Metabolism01:20

Overview of Nitrogen Metabolism

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Nitrogen is a very important element for life because it is a major constituent of proteins and nucleic acids. It is a macronutrient, and in nature, it is recycled from organic compounds and stored in the form of  ammonia, ammonium ions, nitrate, nitrite, or  nitrogen gas by many metabolic processes. Many of these metabolic processes are carried out only by prokaryotes.
The largest pool of nitrogen available in the terrestrial ecosystem is gaseous nitrogen (N2) from the air, but this...
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Estimating Sediment Denitrification Rates Using Cores and N2O Microsensors
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Estimating Sediment Denitrification Rates Using Cores and N2O Microsensors

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Nitrite soaking pretreatment induced initial denitrifying nitrite accumulation.

Ji Zhao1, Yuzhe Wu1, Jimiao Wang2

  • 1School of Environmental Science and Engineering, Qingdao University, Qingdao 266071, China.

Bioresource Technology
|August 6, 2023
PubMed
Summary

Nitrite soaking pretreatment effectively accelerates partial denitrification (PD) startup. This method enhances nitrite accumulation, making PD more feasible for research and applications.

Keywords:
Denitrifying nitrite accumulationMicrobial communityNitrite soakingPartial denitrificationPre-treatment

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Area of Science:

  • Environmental microbiology
  • Water treatment technologies

Background:

  • Partial denitrification (PD) is a method for nitrite production.
  • Slow startup of PD limits its practical application and research.

Purpose of the Study:

  • To investigate nitrite soaking as a pretreatment to accelerate PD startup.
  • To optimize nitrite soaking conditions for enhanced nitrite accumulation.

Main Methods:

  • Pretreatment of microbial communities using nitrite soaking.
  • Varying nitrite concentrations and soaking durations.
  • Monitoring denitrifying nitrite accumulation under different conditions, including high pH and frequent feeding.

Main Results:

  • Nitrite soaking for 9 hours with 10 mg/L nitrite initiated PD with 4.20 mg/L accumulation.
  • Soaking for 6 hours with 20 mg/L nitrite yielded the highest accumulation (4.92 mg/L).
  • High pH (9) and frequent feeding further improved nitrite accumulation.

Conclusions:

  • Nitrite soaking is an effective pretreatment for rapid PD startup.
  • Optimized soaking conditions and operational parameters (pH, feeding) enhance nitrite accumulation.
  • Pretreatment-induced microbial community shifts favor PD over complete denitrification.