A novel electrochemical aptasensor based on polyaniline and gold nanoparticles for ultrasensitive and selective...

Ascorbic acid (AA) is involved in many physiological activities of the body and plays an important role in maintaining and promoting human health. It is also present in many natural and artificial foods. Therefore, the development of highly sensitive and accurate AA sensors is highly desirable for human health monitoring, as well as other commercial application fields. Herein, an ultrasensitive and selective electrochemical sensor based on an aptamer was developed for the determination of AA for the first time. The aptasensor was fabricated by modifying a composite made of polyaniline (PANI) and gold nanoparticles (AuNPs) on a glassy carbon electrode. The morphologies and electrochemical properties of the resulting electrodes were characterized by various analytical methods. The results indicated relatively good electrical conduction properties of PANI for accelerated electron transfer. The modification with AuNPs provided signal amplification, suitable for applications as novel platforms for the sensitive sensing of AA. Under optimized conditions, the proposed aptasensor displayed a wide linear response toward the detection of AA from 1.0 to 1.0 × 10⁵ ng L^-1 coupled with a low detection limit of 0.10 ng L^-1. The sensor also exhibited excellent selectivity and high stability, with at least 2000-fold higher sensitivity than similar previously reported methods. Importantly, the aptasensor exhibited promising properties for the determination of AA in real fruits, vegetables, and infant milk powder, thereby showing potential for food analysis.