Related Experiment Video

Updated: Jul 19, 2025

Direct Restart of a Replication Fork Stalled by a Head-On RNA Polymerase
07:27

Direct Restart of a Replication Fork Stalled by a Head-On RNA Polymerase

Published on: April 29, 2010

13.6K

Disparate requirements for RAD54L in replication fork reversal

Mollie E Uhrig, Neelam Sharma, Petey Maxwell

    Biorxiv : the Preprint Server for Biology
    |August 7, 2023
    PubMed
    Summary

    RAD54L restrains DNA replication fork progression and remodels forks in human cells. It functions distinctly in two RAD51-mediated pathways, suggesting therapeutic potential.

    More Related Videos

    Detection of Homologous Recombination Intermediates via Proximity Ligation and Quantitative PCR in Saccharomyces cerevisiae07:55

    Detection of Homologous Recombination Intermediates via Proximity Ligation and Quantitative PCR in Saccharomyces cerevisiae

    Published on: September 11, 2022

    1.9K
    Inducing a Site Specific Replication Blockage in E. coli Using a Fluorescent Repressor Operator System11:19

    Inducing a Site Specific Replication Blockage in E. coli Using a Fluorescent Repressor Operator System

    Published on: August 21, 2016

    9.0K

    Related Concept Videos

    Restarting Stalled Replication Forks02:37

    Restarting Stalled Replication Forks

    DNA replication is initiated at sites containing predefined DNA sequences known as origins of replication. DNA is unwound at these sites by the minichromosome maintenance (MCM) helicase and other factors such as Cdc45 and the associated GINS complex.The unwound single strands are protected by replication protein A (RPA) until DNA polymerase starts synthesizing DNA at the 5’ end of the strand in the same direction as the replication fork. To prevent the replication fork from falling apart,...
    5.8K
    The DNA Replication Fork01:02

    The DNA Replication Fork

    An organism’s genome needs to be duplicated in an efficient and error-free manner for its growth and survival. The replication fork is a Y-shaped active region where two strands of DNA are separated and replicated continuously. The coupling of DNA unzipping and complementary strand synthesis is a characteristic feature of a replication fork.   Organisms with small circular DNA, such as E. coli, often have a single origin of replication; therefore, they have only two replication...
    36.1K
    Homologous Recombination02:31

    Homologous Recombination

    The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
    50.7K
    The Replisome03:01

    The Replisome

    DNA replication is carried out by a large complex of proteins that act in a coordinated matter to achieve high-fidelity DNA replication. Together this complex is known as the DNA replication machinery or the replisome.
    The synthesis of the leading and lagging strands is a highly coordinated process. To explain this, the “Trombone model” was proposed by Bruce Alberts in 1980. The DNA loop formation starts when a primer is synthesized on the parent lagging strand. The loop grows with...
    33.7K
    Replication in Eukaryotes01:29

    Replication in Eukaryotes

    In eukaryotic cells, DNA replication is highly conserved and tightly regulated. Multiple linear chromosomes must be duplicated with high fidelity before cell division, so there are many proteins that fulfill specialized roles in the replication process. Replication occurs in three phases: initiation, elongation, and termination, and ends with two complete sets of chromosomes in the nucleus.
    Many Proteins Orchestrate Replication at the Origin
    Eukaryotic replication follows many of the same...
    14.0K
    Replication in Prokaryotes01:32

    Replication in Prokaryotes

    DNA replication has three main steps: initiation, elongation, and termination. Replication in prokaryotes begins when initiator proteins bind to the single origin of replication (ori) on the cell's circular chromosome. Replication then proceeds around the entire circle of the chromosome in each direction from the two replication forks, resulting in two DNA molecules.
    Many Proteins Work Together to Replicate the Chromosome
    Replication is coordinated and carried out by a host of specialized...
    25.0K