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Related Experiment Videos

Is duplicate testing for prothrombin time and activated partial thromboplastin time necessary?

A A Keshgegian, J M Mann, J H Cooper

    Archives of Pathology & Laboratory Medicine
    |June 1, 1986
    PubMed
    Summary

    Duplicate testing for prothrombin times (PTs) and activated partial thromboplastin times (APTTs) is often unnecessary. Most coagulation test duplicates show minimal differences, indicating high precision in automated instruments.

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    Human pathology·2000

    Area of Science:

    • Clinical Pathology
    • Hematology
    • Coagulation Diagnostics

    Background:

    • Prothrombin time (PT) and activated partial thromboplastin time (APTT) are critical coagulation assays.
    • Duplicate testing is a common quality control measure in clinical laboratories.
    • The necessity of duplicate PT and APTT testing on automated analyzers requires evaluation.

    Purpose of the Study:

    • To assess the precision of duplicate prothrombin time (PT) and activated partial thromboplastin time (APTT) measurements.
    • To determine the frequency and magnitude of differences in duplicate coagulation test results.
    • To evaluate the clinical necessity of routine duplicate PT and APTT testing.

    Main Methods:

    • Analysis of duplicate results from 1,610 PTs and 1,023 APTTs.

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  • Utilized a widely used automated photo-optical coagulation instrument.
  • Included specimens with coagulation test times from normal to threefold above the reference range.

    • Main Results:

    • Approximately 95% of PT duplicates differed by ≤0.2 s and 95% of APTT duplicates by ≤2.0 s.
    • Approximately 99% of PT and APTT duplicates differed by ≤0.4 s and ≤4.0 s, respectively.
    • A small percentage of specimens showed larger differences, potentially impacting diagnosis or therapy.

    Conclusions:

    • Automated PT and APTT testing demonstrates high precision.
    • Routine duplicate testing may not be necessary for most samples due to instrument reliability.
    • Occasional discrepancies highlight the importance of monitoring for potential diagnostic or therapeutic errors.