Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Perioperative effects of caudal block on pediatric patients in laparoscopic upper urinary tract surgery: a randomized controlled trial.

BMC pediatrics·2019

Same author

Bone formation promoted by bone morphogenetic protein-2 plasmid-loaded porous silica nanoparticles with the involvement of autophagy.

Nanoscale·2019

Same author

Molecular identification guided process design for advanced treatment of electroless nickel plating effluent.

Water research·2019

Same author

Analyzing Complexity and Fractality of Glucose Dynamics in a Pregnant Woman with Type 2 Diabetes under Treatment.

International journal of biological sciences·2019

Same author

Time delay in seeking treatment for first-episode schizophrenia: a retrospective study.

Early intervention in psychiatry·2019

Same author

The S∴π hemibond and its competition with the S∴S hemibond in the simplest model system: infrared spectroscopy of the [benzene-(H<sub>2</sub>S) <sub></sub> ]<sup>+</sup> (<i>n</i> = 1-4) radical cation clusters.

Chemical science·2019

Search research articles

Related Experiment Video

Updated: Jul 19, 2025

Author Spotlight: Optimizing the Rearing Procedure of Germ-Free Wasps

Author Spotlight: Optimizing the Rearing Procedure of Germ-Free Wasps

Published on: July 21, 2023

Optimizing the Rearing Procedure of Germ-Free Wasps.

Zhengyu Zhu¹, Dandan Wang², Yanjun Liu²

¹State Key Laboratory of Integrated Management of Pest Insects and Rodents, Institute of Zoology, Chinese Academy of Sciences; College of Life Sciences, Hebei University.

Journal of Visualized Experiments : Jove

|August 7, 2023

Summary

Optimized aseptic rearing medium for Nasonia wasps simplifies preparation and extends shelf life. This advancement supports robust insect microbiota research and host-microbe interaction studies.

More Related Videos

Embryo Microinjection and Transplantation Technique for Nasonia vitripennis Genome Manipulation

Embryo Microinjection and Transplantation Technique for Nasonia vitripennis Genome Manipulation

Published on: December 25, 2017

An Introduction to Parasitic Wasps of Drosophila and the Antiparasite Immune Response

An Introduction to Parasitic Wasps of Drosophila and the Antiparasite Immune Response

Published on: May 7, 2012

Related Experiment Videos

Last Updated: Jul 19, 2025

Author Spotlight: Optimizing the Rearing Procedure of Germ-Free Wasps

Author Spotlight: Optimizing the Rearing Procedure of Germ-Free Wasps

Published on: July 21, 2023

Embryo Microinjection and Transplantation Technique for Nasonia vitripennis Genome Manipulation

Embryo Microinjection and Transplantation Technique for Nasonia vitripennis Genome Manipulation

Published on: December 25, 2017

An Introduction to Parasitic Wasps of Drosophila and the Antiparasite Immune Response

An Introduction to Parasitic Wasps of Drosophila and the Antiparasite Immune Response

Published on: May 7, 2012

Area of Science:

Microbiology
Entomology
Insect Rearing Technology

Background:

Aseptic rearing is crucial for studying insect microbiota and host-microbe interactions by controlling microbial environments.
Nasonia wasps are valuable insect models due to their short lifespan and genetic variability.
Existing Nasonia rearing media (NRM) present challenges including complex preparation, contamination risks, and short storage duration.

Purpose of the Study:

To optimize Nasonia rearing medium (NRM) for improved preparation, storage, and usability.
To enhance the survival rate and health of aseptic Nasonia for microbial research.

Main Methods:

Optimized preparation tools, storage conditions, and component ratios for NRM.
Evaluated NRM stability at -20 °C for extended periods.
Assessed the impact of the optimized NRM on aseptic Nasonia survival and health.

Main Results:

The optimized NRM can be stored at -20 °C for at least 3 months.
The new method eliminates contamination risks during the feeding of sterile wasps.
Improved survival rates and health levels were observed in aseptic Nasonia reared with the optimized medium.

Conclusions:

The optimized NRM significantly improves the practicality and reliability of aseptic Nasonia rearing.
This advancement facilitates more effective research into insect microbiota and host-microbe interactions using Nasonia models.