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Cyclodextrin glucanotransferase: fundamentals and biotechnological implications.

Dimple S Pardhi¹, Khushbu J Rabadiya¹, Rakeshkumar R Panchal¹

¹Department of Microbiology and Biotechnology, University School of Sciences, Gujarat University, Ahmedabad, 380009, Gujarat, India.

Applied Microbiology and Biotechnology

|August 7, 2023

Summary

Cyclodextrin glucanotransferase (CGTase) enzymes transform starches into cyclodextrins via transglycosylation. This review compiles bacterial CGTase sources, properties, and cyclodextrin applications in various industries.

Keywords:

Bacillus spp.CGTase Cyclization Cyclodextrins Intermolecular transglycosylation reactions

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Area of Science:

Enzymology and Biotechnology
Microbial Biochemistry

Background:

Cyclodextrin glucanotransferase (CGTase) is a GH13 α-amylase family enzyme.
CGTase catalyzes cyclization, coupling, disproportionation, and hydrolysis of α-1,4-glucans.
Cyclodextrins form inclusion complexes, finding broad industrial applications.

Purpose of the Study:

To compile bacterial sources, biochemical properties, production conditions, and structures of CGTases.
To review CGTase production by Bacillus spp. and other bacterial genera.
To provide insights into cyclodextrin properties and biotechnological applications.

Main Methods:

Literature review of CGTase and cyclodextrin research.
Compilation of data on bacterial CGTase producers, focusing on Bacillus spp.
Analysis of CGTase biochemical properties and production parameters.

Main Results:

Bacillus genus accounts for ~90% of CGTase producers.
Alkaliphilic bacteria under submerged fermentation are optimal for CGTase production.
CGTases exhibit five distinct domains (A-E) with variable structures.

Conclusions:

CGTase is a versatile enzyme with significant biotechnological potential.
Diverse bacterial genera contribute to CGTase production, with Bacillus spp. being predominant.
Cyclodextrins offer unique properties for applications in food, pharmaceuticals, and chemical industries.